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Profiling urinary steroids. A reliable procedure


, : Profiling urinary steroids. A reliable procedure. Clinica Chimica Acta; International Journal of Clinical Chemistry 38(1): 103-111

A relatively simple method of gas chromatography, permiting the quantitative estimation of the most important urinary 17-ketosteroids and 17-OH corticosteroids, is reported. The procedure consists in enzyme hydrolysis, dichloromethane extraction, and (without any further purification) gas-liquid chromatography of the steroids as their o-methyloxime-trimethyl silyi ether (MO-TMS) derivatives. The method is shown to be useful in clinical studies.

US$19.90

PMID: 5031774

DOI: 10.1016/0009-8981(72)90214-8


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