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Conservation of pathogenic mycoplasma antigens


, : Conservation of pathogenic mycoplasma antigens. Israel Journal of Medical Sciences 20(10): 916-919

The protein profiles of different mycoplasmas are generally species-specific; however, several pathogenic species share certain biologic properties and/or virulence factors. We compared species of human, avian and animal origin to determine if there was evidence for conservation of key antigens. Log-phase cultures of Mycoplasma pneumoniae, M. genitalium, M. gallisepticum and M. pulmonis were subjected to SDS-gel electrophoretic analysis. Protein profiles were compared with silver-stained gels. To examine antigenic cross-reactivity, Western blots were prepared by transferring separated proteins to nitrocellulose filters and incubating these with homotypic and heterotypic antisera. Localization of cross-reacting antigens was done by immunoradioautography using 125I-labeled antiglobulins. Several two-way cross-reactions were observed among M. pneumoniae, M. genitalium, and M. gallisepticum; M. pulmonis shared no significant cross-reactions with the other three species. Preliminary evidence suggests that the cross-reactivity was due to conservation of certain antigenic determinants, although the overall protein composition of the four species was different.

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PMID: 6210266


Other references

Nam, H.W.o; Im, K.S.im; Baek, E.J.ng; Choi, W.Y.ung; Cho, S.Y.ll, 1996: Analysis of antigenic domain of GST fused major surface protein fragments of Toxoplasma gondii. Antigenic domain of major surface protein (p30) of Toxoplasma gondii was analyzed after polymerase chain reaction (PCR) of its gene fragments. Hydrophilic or hydrophobic moiety of amino acid sequences were expressed as glutathione S-transferase (G...

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Price M.V.; Heinz K.M., 1984: Effects of body size seed density and soil characteristics on rates of seed harvest by heteromyid rodents. Although it is well established that coexisting heteromyid rodent species forage in different microhabitats, the basis for divergent microhabitat choice is not yet known. One possibility is that seed harvest rates differ among microhabitats, and e...

Rosenthal, W.; Gerik, T.; Pendleton, B.T.etes, G.M.kela, M.S.one, N., 1989: Sorghum midge control using a plant. Paper American Society of Agricultural Engineers: er 1989 (89-4043)

Timms, P., 1981: Inhibition of Babesia spp. by plastics. Five commercially available plastic containers [used in vaccine preparation] were compared with glass for toxicity toward B. rodhaini and B. bigemina. Comparisons were made by using infectivity tests in mice (B. rodhaini) and cattle (B. bigemina)....