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Rapid cultivation of mycobacteria with a rotating incubator


, : Rapid cultivation of mycobacteria with a rotating incubator. Zeitschrift für Erkrankungen der Atmungsorgane 158(1-2): 130-141

A method is described which allows a rapid primary culture of mycobacteria. The basic principle is a rotating incubator fitted with special cultures tubes and a synthetic liquid culture medium. So it is possible to accelerate the growth and multiplication and to get instance an average generation time of 10 hours in M. tuberculosis. After conventional decontamination of the sputa with Nekal-BX and an incubation time of 1, 2 and 4 weeks the grown mycobacteria are proved by microscopy with a special fixation technique. Acridin-orange is used for staining. The conditions for application of the rapid culture method are discussed.

US$29.90

PMID: 6803453


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Tindholdt, T.Tind.; Mesic, H.; Tønseth, K.Alexander.; Harbo, S.Olaus., 2005: 40 years of silicone breast implants. It is estimated that approximately 50,000 Norwegian women have silicone breast implants following breast augmentation or breast reconstruction. Their numbers are growing and doctors will more often be confronted with the health problems related to...

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