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A mini-review on the technique of insect glutathione S-transferases purification


, : A mini-review on the technique of insect glutathione S-transferases purification. Chinese Bulletin of Entomology 44(6): 810-814

The main purification methods of insect glutathione S-transferases include fractionation, chromatography and electrophoresis. Fractionation usually is used before chromatography and its purification factor is lower, while purification factor of chromatography is higher. Electrophoresis is used as an assistance purification method. Using these methods, the highest purification factor of insect glutathione S-transferase is 1 138 fold ( Ma et al. purified glutathione S-transferases of Asian core borer Ostrinia furnacalis by using chromatography and electrophoresis). At last, the applications of purification of insect glutathione S-transferases are discussed.

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