+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A Protein Subunit Of Bromegrass Mosaic Virus

, : A Protein Subunit Of Bromegrass Mosaic Virus. Journal of Molecular Biology 8: 314-323

The protein and RNA of bromegrass mosaic virus were separated from each other by dialysis of the virus against 1 [image]-calcium chloride. In this medium the virus is degraded, the RNA is quantitatively precipitated and there remains a supernatant solution of viral protein. The protein obtained in this way was soluble in various buffers in the pH range 5.5 to 6.5 and exhibited a single symmetrical sedimenting boundary in the ultracentrifuge. Its molecular weight was 40,000 [plus or minus] 2,000 as determined by the Ehrenberg method of approach to sedimentation equilibrium. Amino acid analyses indicated that there were about 52 lysyl plus arginyl residues per molecular weight of 40,000. Tryptic hydrolysis of urea-denatured protein yielded 23 soluble peptides and an insoluble residue amounting to about 20% by weight of the intact protein. From the number of soluble tryptic peptides and an assumed number of tryptic peptides in the insoluble residue it was concluded that the molecular weight of the protein subunit of bromegrass mosaic virus was slightly greater than 20,000 and that the protein moiety obtained by treatment with 1 [image]-CaCl2 was a relatively stable dimer of the subunit. Partial separation of the soluble peptides resulting from tryptic hydrolysis was achieved on a preparative scale by column chromatography.


PMID: 14126299

DOI: 10.1016/S0022-2836(64)80140-6

Other references

Davies, H.; Williams, A.E.; Morgan, W.A., 1970: The effect of mildew and leaf blotch on yield and quality of cv. Lior Italian Ryegrass. Plots were sprayed with a fungicide (zineb, dimane or ZnSO4) at 10-day intervals, and 4 cuts were taken during the season. Disease assessments for Erysiphe graminis and Rhynchosporium spp. were made at harvest on the basis of leaf area affected. C...

Berliner, J.A.; Leitinger, N.; Vora, D.; Teng, P.; Territo, M., 1997: The role of oxidized lipids in regulation of adhesion molecule expression. Journal of Molecular & Cellular Cardiology 29(7): A257

Kuimova, T.; Paronyan, A., 1973: Curdling and peptonization of milk as a taxonomic property of actinomycetes. Microbiology Transl 1974, 42 (6) 988-989

Amir M.K.ynia; Eduardo Kausel, 1991: Dynamics of piles and pile groups in layered soil media. A general formulation is presented for the dynamic response analysis of piles and pile groups in a layered halfspace. Green's functions for layered media, evaluated numerically by the application of Integral transform techniques, along with a...

Zhao Ying; Tong YanAn; Zhao HuBing, 2006: Effects of different N levels on yield and N utilization and balance in summer maize. A field trial was conducted with summer maize cv. Shendan 10 in Yangling, Shaanxi, China in 2004, to study the effects of different N levels (0, 125, 250 and 375 kg N/ha) as urea on yield, N use efficiency and N balance. The maize yield increased...

Corwin, E., 1975: Opening the closed door. Fda Consumer 9(3): 24-27

Burgess; Barrow; Gao; Heard; Glenn, 1999: Carotenoid glycoside esters from the thermophilic bacterium meiothermusruber. The thermophilic bacterium Meiothermus ruber produces a series of carotenoid glycoside esters. The major carotenoid has been identified as 1'-beta-glucopyranosyl-3,4,3',4'-tetradehydro-1', 2'-dihydro-beta,psi-caroten-2-one...

Schmidt, O.; Scrimgeour, C.M.; Curry, J.P., 1999: Carbon and nitrogen stable isotope ratios in body tissue and mucus of feeding and fasting earthworms (Lumbricus festivus). Natural abundance stable isotope techniques were used to estimate carbon and nitrogen turnover rates in body tissue and mucus of earthworms. Isotope ratios of carbon ( delta 13C) and nitrogen ( delta 15N) were monitored simultaneously in body tiss...

Ellegast, H.; Meixner, M., 1968: X-ray diagnostic contribution on alkaptonuria. Radiologia Clinica et Biologica 37(6): 331-337

Ostrowski-De-Nunez, M., 2002: Digenean trematodes of crocodiles collected by Johann Natterer in Brazil, deposited in the Natural History Museum, Vienna. The helminths from six vials in the Collection of the Natural History Museum, Vienna, were mounted as permanent preparations and the following species were found: Stephanoprora nattereri sp.n., Stephanoprora sp., Caballerotrema sp., Echinostoma sp...