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A pure culture technique for studying the infection phenomenon of oak roots by Armillaria mellea

, : A pure culture technique for studying the infection phenomenon of oak roots by Armillaria mellea. Proc Iowa Acad Sci 67: 126-132

Oak seedlings were grown in a pure culture of A. mellea in large test tubes. A layer of white quartz sand, in which an acorn was planted, covered a PDA medium containing rhizomorphs of the fungus. After 4 months growth, roots from the oak seedlings were extracted from the agar, washed, fixed in FAA, sectioned, stained, and examined microscopically. Abnormal areas of the tap roots showed epidermis demarcated by a black layer of suberized cells. One section showed this suberized layer broken by penetration of a foreign object. The cortex around this penetration point-was disrupted and stained a dark brown color. The affected area was demarcated from healthy cells by a layer of cork, 3 cells thick. This technique will enable the development of more critical studies on this infection phenomenon.


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