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Acid phosphatase content of nuclei of chicken erythrocytes

, : Acid phosphatase content of nuclei of chicken erythrocytes. Proc Soc Exp Biol And Med 54(1): 22-24

Nuclei isolated from chicken erythrocytes by the method of Dounce and Lan were investigated for acid phosphatase activity. Acid phosphatase was present in an amt. sufficient to cause liberation of 0.0025 to 0.0030 mg. of phenol/hr. from di-sodium phenyl phosphate at pH 5.0 and 25[degree]C. The activity of the enzyme dropped rather suddenly to a negligible value after incubation of the nuclei with the substrate for about 2 hrs.


DOI: 10.3181/00379727-54-14287

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