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Alternative promoter usage generates multiple evolutionarily conserved isoforms of Drosophila DOA kinase


, : Alternative promoter usage generates multiple evolutionarily conserved isoforms of Drosophila DOA kinase. Genesis 46(3): 132-143

The unique LAMMER (or Clk) protein kinase of Drosophila is encoded at the Doa locus. To better understand the pleiotropic effects of Doa mutations, we describe the structure and expression of the multiple RNA and protein products of the locus, as well as their evolutionary conservation among Drosophila. The gene produces at least six different protein isoforms, primarily through alternative promoter usage, generating kinases with virtually identical catalytic domains but variable N-terminal noncatalytic domains. The single known alternative splicing event generates a kinase with the insertion of six additional amino-acids in the catalytic domain. Two independent predicted genes nested within Doa introns actually encode additional alternative N-termini of the locus. An alternative polyadenylation site utilized exclusively during early embryogenesis generates a transcript with a short half-life, apparently to ensure a "burst" of kinase expression at the onset of development. Ecdysone induction of Doa transcripts affects all isoforms during pupariation. Finally, extensive conservation of amino-acid sequences of both the catalytic and N-terminal noncatalytic exons observed in alignments between D. melanogaster exons and the genome sequences of 11 other Drosophila species suggest that the multiple isoforms serve important and non-redundant functions.

US$19.90

PMID: 18327787

DOI: 10.1002/dvg.20374


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Yan, N.; Zhu, B-Cai.; Wang, Y-Feng., 2009: Recent advances on sex determining mechanisms of Microtus mandarinus. Most mammalian sex determination belongs to male heterogametic type of genetic sex determination. Sex determination of most mammals depends on the Y chromosome. SRY gene is the testis-determining factor on Y chromosome of most mammals, which is th...

Donaldson, G.C.; Rintamaki, H.; Nayha, S., 2001: Outdoor clothing: Its relationship to geography, climate, behaviour and cold-related mortality in Europe. It has been suggested, that the inhabitants of northern European regions, who experience little cold-related mortality, protect themselves outdoors by wearing more clothing, at the same temperature, than people living in southern regions where suc...

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Rosen, H.M.; Slivjak, M.J.; McBrearty, F.X., 1987: Delayed microcirculatory hyperpermeability following perfusion washout. Recent experimental data have demonstrated improved flap survival following perfusion washout with a synthetic, chemically defined, mammalian plasma. In an effort to define the physiology responsible for the efficacy of perfusion, the method of &q...

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