geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

An improved technique for analysis of free fatty acids in butteroil and Provolone cheese


, : An improved technique for analysis of free fatty acids in butteroil and Provolone cheese. J Dairy Sci 50(3): 285-291

A method is described for isolating the free fatty acids (FFA) of butter oil and Provolone cheese using the silicic acid-KOH column of McCarthy and Duthie. The FFA were eluted from the column with phosphoric acid in diethyl ether. After addition of methanol the FFA and excess phosphoric acid were titrated with methanolic KOH. The potassium phosphate salts were sedlmented and the supernatant was evaporated to dryness under N. Experiments indicated no saponlficatlon of triglycerldes and no esterification of FFA during the above manipulations. Butyl esters were prepared using sulfuric acid as a catalyst and esters from butyrate through llnolenate were estimated with gas-liquid chromatography using heptanoic and hepta-decanoic acids as internal standards. FFA were determined in 3 different samples of butter oil and in Provolone cheese. In butter oil the short-chain FFA were low and the long-chain FFA were high in concentration. In Provolone cheese the short-chain FFA were extremely high. Butyric acid was highest in concentration of all the FFA.

US$29.90


Other references

Jagoe, R.B., 1952: Photoperiodism of Oryza sativa in Malaya. Rice is typically a "short-day" plant in Malaya since it flowers successfully only during Oct. to Feb., even though days are only slightly shorter than during May to Aug. However, some vars. produce good crops during the "off-season...

Voronina, T.A.; Smirnov, L.D.; Tilekeeva, U.M.; Diumaev, K.M., 1986: The role of GABA-benzodiazepine receptor complex in affecting the anxiolytic effect of 3-hydroxypyridines--new non-benzodiazepine tranquilizers. The experiments on rats, using conflict situation method, have established that anxiolytic effect of 3-hydroxypyridine derivative is removed by bicucullin and picrotoxin, but not by Ro 5-3663, specific antagonist of benzodiazepine receptors Ro 15-...

Alexander, M.Yvonne., 2009: RANKL links arterial calcification with osteolysis. Circulation Research 104(9): 1032-1034

Molotch H., 1976: Santa Barbara; oil in the velvet playground.; 2. Pages 364-377 1976

Lindstrom, T.B.rgstrand, E., 1979: The habitat of perch Perca fluviatilis L. on the outskirts of its Swedish distribution, lakes and lake reservoirs. Report Institute of Freshwater Research Sweden: 8) 55-76

Buitrago V., F., 2006: Conceptual aspects on connectivity in Mesoamerica Aspectos conceptuales de la conectividad en Mesoamerica. Connectivity can be defined as the maintenance of the functions and ecological process that make possible the survivalship of species in a long period of time. Connectivity is everything what happens in the ecosystems that allow ecological process...

Howes L.G.; Summers R.J.; Rowe P.R.; Louis W.J., 1983: Simultaneous determination of 3 4 di hydroxyphenyl ethylene glycol 3 4 di hydroxyphenyl acetic acid and catecholamines in brain tissue by high performance liquid chromatography with electrochemical detection. An assay is described for the simultaneous determination of the deaminated metabolites 3,4-dihydroxyphenylethylene glycol (DHPG) and 3,4-dihydroxyphenylacetic acid (DOPAC), together with noradrenaline [norepinephrine] dopamine and adrenaline epine...

Teleb, H.M.; Fakhry, F.M., 1988: Aflatoxin B1 residue in broiler and its effect on fat metabolism. A total of 125 one-day-old Hubbard chickens were divided into 5 groups and were fed diets containing graded levels of aflatoxin B1 at the rate of 0, 100, 250, 500 and 750 p.p.b. Residues of aflatoxin were detected in liver, heart, breast muscle an...

Ye, D.; Xu, Z-nan.; Cen, P-lin., 2008: Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue (MIL-C) by Streptoverticillium rimofaciens ZJU 5119. Cytosine-substituted mildiomycin analogue (MIL-C) was produced effectively by supplementing cytosine into the culture of Streptoverticillium rimofaciens. In order to improve the yield of MIL-C, statistically-based experimental designs were applied...

Steinberg, M.S.; McNutt, P.M., 1999: Cadherins and their connections: adhesion junctions have broader functions. Cadherins — a family of cell–cell adhesion molecules — are linked to the actin cytoskeleton via intervening proteins. Recent results address molecular explanations for observed cadherin behavior, point to signals that regulate ad...