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Analysis of 5-HTP-C-14 and its metabolites


, : Analysis of 5-HTP-C-14 and its metabolites. Analytical Biochemistry 11(3): 467-472

A method was developed for the analysis of 5-hydroxytryptophan (5-HTP)-C14 and its metabolites in rat liver homogenate. 5-HIAA and neutrals were extracted from the acidified salt-saturated incubation mixture. 5-hydro-xylndoleacetic acid (5-HIAA) was separated from the neutrals by extraction into pH 10 salt-saturated borate buffer. After making the residual incubation mixture alkaline, serotonin was separated by acetylation and extraction into isopropyl acetate. The residual aqueous solution was acidified and the acetylated 5-HTP extracted into isopropyl acetate. The method was used to study the alteration of DL-5-HTP-C14 metabolism in rat liver homogenate by NAD and NADH. In the unfortified incubation mixture, 5-hydroxyindoleacetaldehyde was the major product; in the NAD incubation mixture, 5-HIAA was the major product; in the NADH incubation mixture, 5-hydroxytryptophol was the major metabolite.

US$19.90

PMID: 4285716

DOI: 10.1016/0003-2697(65)90066-7


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