+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Analysis of 5-HTP-C-14 and its metabolites

, : Analysis of 5-HTP-C-14 and its metabolites. Analytical Biochemistry 11(3): 467-472

A method was developed for the analysis of 5-hydroxytryptophan (5-HTP)-C14 and its metabolites in rat liver homogenate. 5-HIAA and neutrals were extracted from the acidified salt-saturated incubation mixture. 5-hydro-xylndoleacetic acid (5-HIAA) was separated from the neutrals by extraction into pH 10 salt-saturated borate buffer. After making the residual incubation mixture alkaline, serotonin was separated by acetylation and extraction into isopropyl acetate. The residual aqueous solution was acidified and the acetylated 5-HTP extracted into isopropyl acetate. The method was used to study the alteration of DL-5-HTP-C14 metabolism in rat liver homogenate by NAD and NADH. In the unfortified incubation mixture, 5-hydroxyindoleacetaldehyde was the major product; in the NAD incubation mixture, 5-HIAA was the major product; in the NADH incubation mixture, 5-hydroxytryptophol was the major metabolite.


PMID: 4285716

DOI: 10.1016/0003-2697(65)90066-7

Other references

Riggio, O.; Nicolini, G.; Angeloni, S.; Rinaldi, V.; Attili, A.F.; Merli, M., 2001: Natural history of portal hypertensive gastropathy in patients with liver cirrhosis. Hepatology 34(4 Pt 2): 536A, October

Hirose, A.; Ema, M.; Hasegawa, R., 2004: Safety evaluation of chemicals using bibliographic databases for their effects on gene expression profiles. Kokuritsu Iyakuhin Shokuhin Eisei Kenkyujo Hokoku 121): 124-127

Alheid, G.F.; Schulkin, J.; Epstein, A.N., 1992: Amygdala pathways involved in sodium appetite in the rat, anatomical considerations. Society for Neuroscience Abstracts 18(1-2): 1231

Likholat, T.V.; Dubrovskii, N.G., 1985: Photosynthesis, nitrogen fixation ability and yield of lupin plants grown from seeds with deteriorated sowing qualities as affected by phytohormone treatment. The assessment of yield and physiological characteristics of lupin [Lupinus luteus] plants grown from seed exposed to unfavourable conditions (41 degrees C and 100% RH) revealed decreased photochemical activity of chloroplasts, photophosphorylatio...

Watson, J.P., 1987: Sex therapy still in demand. British Journal of Hospital Medicine 38(2): 147-149

Max, B., 1992: This and that: sequential theories for stomach cancer and the multiple applications of isotopic ratios. Trends in Pharmacological Sciences 13(4): 136-140

Fuger, M.; Merdariu, D.; Maurey, H.; Kaminska, A.; Chéron, G., 2015: Relevance of electroencephalography in infants presenting to an emergency department who have had an apparent life-threatening event. Neurological causes are common diagnoses for apparent life-threatening events in infants. The objective of this study was to evaluate the relevancy of electroencephalography performed after an apparent life-threatening event. A retrospective study...

Durand, M.; Bourgeon, R.; Guntz, M.; Pietri, H., 1961: The value of transparietal splenoportography in hydatid cysts of the liver. Journal de Radiologie, D'electrologie, et de Medecine Nucleaire 42: 743-756

Jubb, G.L.J.; Obourn, T.H.; Petersen, D.H., 1978: Pilot pest management program for grapes in Erie County, Pennsylvania. During the summers of 1976-1977, a pilot pest management program [major pests are the grape berry moth, Endopiza viteana Clemens, the redbanded leafroller, Argyrotaenia velutinana (Walker), the European red mite, Panonychus ulmi (Koch) and a leafh...

Kaplan, M.H.; Boyer, S.N.; Grusby, M.J., 1996: Genomic organization of the murine CTL lipase gene. Murine cytotoxic T-lymphocyte (CTL) lipase was originally identified as an IL-4-inducible gene in CD8-positive T cells. To further our understanding of both the function and the regulation of CTL lipase in T cells, we have cloned and characterized...