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Application of solid-phase extraction coupled with freezing-lipid filtration clean-up for the determination of endocrine-disrupting phenols in fish

, : Application of solid-phase extraction coupled with freezing-lipid filtration clean-up for the determination of endocrine-disrupting phenols in fish. Analytica Chimica Acta 603(1): 67-75

An analytical method has been developed for the determination of endocrine-disrupting phenols (eight alkylphenols and bisphenol A) in fish samples. The extraction of nine phenols from fish samples was carried out by ultrasonification. After the extraction, high levels of lipids were removed by freezing-lipid filtration instead of the traditional methods of column chromatography or saponification. During freezing-lipid filtration, about 90% of the lipids were eliminated without any significant loss of phenolic compounds. For further purification, hydrophilic-lipophilic balanced copolymer (HLB) sorbent with a poly(divinylbenzene-co-N-vinylpyrrolidone) phase and Florisil-solid-phase extraction (SPE) cartridges were used to eliminate the remaining interferences. Silyl-derivatization, with N,N '-methyl-(tert-butyldimethylsilyl) trifluoroacetamide (MTBSTFA), was applied to enhance the sensitivity of detection of phenolic compounds. Quantification was performed by gas chromatography/mass spectrometry (GC/MS)-selected ion monitoring (SIM) mode, using deuterium-labeled internal standards. Spiking experiments were carried out to determine the recovery, precision and detection limit of the method. The overall recoveries ranged between 70 and 120%, with relative standard deviations of 3-17% for the entire procedure. The detection limits of the method for the nine phenols ranged from 0.02 to 0.41 ng g(-1). The method provided simultaneous screening and accurate confirmation of each phenol when applied to biological samples.


PMID: 17950059

DOI: 10.1016/j.aca.2007.09.045

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L.X.ngLiang; Zhang ZhiYong; L.H.ngBin (), 2006: Investigation of the number of malaria reports and actual cases in Meng La County, Yunnan Province. This study assessed the failure in reporting malaria patients in Meng La County to provide a scientific basis for disease control measures and to offer baseline data for estimating the effects of a global fund program for disease control. Data on...

Anonymous, 1986: Infant growth and obesity in Samoa. A discussion of recent reports on infant growth and obesity incidence in American Samoa concludes that the birth weight and growth of Samoan infants are greater than for infants of other countries, regardless of feeding regimen or rural-urban diff...

Degrange, C.; Bouvet, J., 1963: Odonates du Vaucluse. Travaux du Laboratoire d'Hydrobiologie et de Pisciculture Universite Grenoble, 54 & 55: 143-153

Cadranel J.L.; Milleron B.J.; Mayaud C.M.; Akoun G.M., 1990: Lymphocyte alveolitis in amiodarone associated pneumonitis. American Review of Respiratory Disease 141(4 PART 2): A312

Malyarova, L.K., 1966: New experimental material on the biological action of trichloroethylene. Tr Ufim Nauch Issled Inst Gig Prof Zabol 3: 312-315

W.J.M., 1988: Nuclear phosphoprotein changes during hl 60 cell differentiation into monocytes. Faseb Journal: Abstract 4187

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Wiktorowicz Belzyt, E.; Wojak, I.; Janicka, G., 1997: Cefazolin, cefamandole and ceftazidime susceptibility of methicillin-resistant and methicillin-susceptibile staphylococci. 365 S. aureus and 165 Coagulase Negative Staphylococci were tested for susceptibility to methicillin, cefazolin, cefamandole and ceftazidime by standard broth microdilution and disc method. In staphylococci resistance to methicillin normally paral...

Polanski, M.; Okon, K.; Przbylo, R.; Frasik, W., 1998: Cardioprotective properties of hydrophilic pollen extract. Pharmacological effects of hydrophilic pollen extract (HPE) are unknown. The results of our previous studies suggest their cardioprotective influence. The purpose of the present study was to evaluate biochemical and morphological effects of HPE in...

Wostmann, B.S.; Gordon, H.A., 1958: Electrophoretic studies on serum proteins of young germfree, conventional and antibiotic treated conventional chickens. The effect of feeding procaine penicillin G (50mg/kg diet) from birth on the electrophoretic pattern of the serum of young conventional chickens was studied and compared with data obtained from germfree animals. The effect on the morphological cha...