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Binding of xenobiotics to the estrogen receptor of spotted seatrout A screening assay for potential estrogenic effects


, : Binding of xenobiotics to the estrogen receptor of spotted seatrout A screening assay for potential estrogenic effects. Marine Environmental Research 35(1-2): 147-151

Several xenobiotics that bind to mammalian estrogen receptors (ER) were tested for their ability to bind to the hepatic ER of a marine fish, the spotted seatrout (Cynoscion nebulosus). Incubation of cytosolic extracts with the antiestrogens clomiphene, tamoxifen, and nafoxidine caused displacement of 3H-estradiol from its receptor. Kepone (chlordecone) also bound to seatrout ER, but it had a lower affinity than that observed with mammalian ER. Preliminary results indicated that these compounds have antiestrogenic or estrogenic actions in the spotted seatrout. However, several DDT derivatives and PCB mixtures failed to displace estradiol from the spotted seatrout ER, even though they have been shown to bind to mammalian ER. The lack of binding to spotted seatrout ER by several of the xenobiotics may be due to structural differences between ER in spotted seatrout and in mammals. These results indicate that the spotted seatrout ER assay can be used for screening xenobiotics for possible estrogenic activity in teleosts.

US$19.90

DOI: 10.1016/0141-1136(93)90029-y


Other references

Gao, J.; Niklason, L.; Langer, R., 1998: Surface hydrolysis of poly(glycolic acid) meshes increases the seeding density of vascular smooth muscle cells. A procedure for surface hydrolysis of poly(glycolic acid) (PGA) meshes was developed to increase cell seeding density and improve attachment of vascular smooth muscle cells. Hydrolysis of PGA in 1N NaOH transformed ester groups on the surface of P...

Chayet L.; Rojas M.C.; Cori O.; Bunton C.A.; Mckenzie D.C., 1984: Complexes of bivalent cations with neryl and geranyl pyrophosphate their role in terpene biosynthesis. Kinetic analysis of the nonenzymic solvolysis of neryl and geranyl pyrophosphate (NPP and GPP, respectively) showed that the dissociation constants of the bis-metallic complexes with Mg2+ and Mn2+ were larger for NPP than for GPP by .apprx. 1 orde...

Joniec, I.; Ciesielska, A.; Kurkowska-Jastrzebska, I.; Przybylkowski, A.; Czlonkowska, A.; Czlonkowski, A., 2010: Age- and sex-differences in the nitric oxide synthase expression and dopamine concentration in the murine model of Parkinson's disease induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. Parkinson's disease (PD) is an age- and sex-related neurodegenerative disorder of unknown aetiology. The involvement of nitric oxide synthase (NOS) in the etiopathogenesis of PD is quite well documented. We decided to examine changes in dopam...

Xu, D.; Li, Y.; Arcaro, M.; Lackey, M.; Bergmann, A., 2005: The CARD-carrying caspase Dronc is essential for most, but not all, developmental cell death in Drosophila. The initiator caspase Dronc is the only Drosophild caspase that contains a caspase activation and recruitment domain (CARD). Although Dronc has been implicated as an important effector of apoptosis, the genetic function of dronc in normal developm...

Sutoo D.; Akiyama K.; Yabe K., 1990: Microphotometric atlas of rat brain 2 tyrosine hydroxylase distribution. Japanese Journal Of Pharmacology. 52(suppl. 1):

Kehoe, R.A., 2012: Medical education, research and practice in relation to industrial health. Medical Press 215: 41-45

Latif, M.A.; Schleicher, D.R.G.; Schmidt, W.; Niemeyer, J., 2013: Black hole formation in the early universe. Supermassive black holes with up to a $\rm 10^{9} M_{\odot}$ dwell in thecenters of present-day galaxies, and their presence has been confirmed at z$\geq$ 6. Their formation at such early epochs is still an enigma. Differentpathways have been sugg...

Lodge, G.A.; Sarkar, N.K.; Kramer, J.K.G., 1978: Fat deposition and fatty acid composition in the neo natal pig. Male Yorkshire pigs (30) from 6 suckled litters were killed in equal numbers at 0, 7, 14, 21 or 28 days of age. Blood glucose level increased by 32% in the 1st wk of life but changed little thereafter; .beta.-hydroxybutyrate level peaked at 14 day...

Orlikov G.A.; Shtofer G.G., 1969: Thrombo elastographic index i as a new index of the thrombo elastogram. Terapevticheskii Arkhiv 41(8): 84-87

Wang, A.; Turner, S.; Van Aert, S.; Van Dyck, D., 2014: An alternative approach to determine attainable resolution directly from HREM images. The concept of resolution in high-resolution electron microscopy (HREM) is the power to resolve neighboring atoms. Since the resolution is related to the width of the point spread function of the microscope, it could in principle be determined fro...