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Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects

, : Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects. Redox Report 13(1): 17-22

To compare the effects of ionising radiation on leukocytes from breast cancer patients and healthy subjects ex vivo, the level of NF-kappaB and the antioxidant enzymes manganese-containing superoxide dismutase (Mn-SOD), copper/zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) in combination with flow cytometric analysis of CD4+ lymphocytes was performed. The level of Mn-SOD protein was significantly increased in the breast cancer study group both before (P < 0.001) and after (P < 0.001) irradiation when compared with healthy subjects. Measurements in parallel indicated that the level of CAT protein was significantly higher in the breast cancer study group after irradiation (2 Gy [P < 0.001] and 9 Gy [P < 0.05]) when compared with healthy subjects. Although the initial number of lymphocytes in the blood of breast cancer patients was not different from healthy subjects, the percentage of apoptotic CD4+ cells was significantly (P < 0.001) lower both before and after irradiation indicating that cell culture conditions induced radioresistance of CD4+ cells in the blood of breast cancer patients. The data presented in this current study indicate that brief ex vivo culture of peripheral blood leukocytes potentiates oxidative stress imposed by a breast cancer tumour.


PMID: 18284847

DOI: 10.1179/135100008X259088

Other references

Limuti, C.; Latart, D.; Mauck, J.; Schaeffer, J.; Warren, H.; Winterkorn, R., 1993: Performance of the Kodak Ektachem clinical chemistry slide in the determination of urinary protein. Clinical Chemistry 39(6): 1135-1136

Elaev N.R.; Ryzhakov A.V., 1986: Products of alkaloid oxidation with blood plasma hemoprotein. Hemoprotein isolated earlier from blood plasma was shown to inactivate alkaloids by transforming them into N-oxides.

Lambe, L.M., 1906: Notes on the fossil Corals collected by Mr. A. P. Low, at Beechy Island, Southampton Island, and Cape Chidley in 1904. Unknown

Anonymous, 1978: Intensification of agricultural production for improvement of the environment. Statni statky1(12): 270-271

Ruiter, J.P.N.; Van Maldegem, B.; Wijburg, F.; Wanders, R.J.A., 2002: A new, simple, non-radiometric assay for glutaryl-CoA dehydrogenase and its application to the rapid diagnosis of glutaric aciduria type I using fibroblasts and/or lymphocytes. Journal of Inherited Metabolic Disease 25(Supplement 1): 54, July

Lidbeck, J.; Månson, J.C., 1979: Staining of granulopoietic agar colonies with a modified Papanicolaou technique. The aceto-orcein method of staining agar cultured granulopoietic marrow cells has not been entirely satisfactory. In staining whole colonies, the original colony structure is lost and the colony cells have blurred cytoplasm. A slight modification...

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Monti, F.; Tonetti, L.; Bitti, P.Enrico.Ricci., 2014: Effectiveness of psychological treatments delivered at a counseling service for students. This study adds to the international literature on the assessment of the effectiveness of psychotherapies delivered by university counseling centers. The present study evaluated the effectiveness of psychotherapy in 226 students (179 women, 47 men...

Perrin, D.D.A.marego, W.L.F.; Kerrigan, J.E.Reviewer; Patil, G.S.Reviewer; Powers, J.C.Reviewer, 1991: Purification of laboratory chemicals (book review). American Journal of Physiology 261: 57

Hilsenhoff W.L., 1988: Seasonal correction factors for the biotic index. For evaluation of streams with the biotic index (BI), samples of arthropods should be collected in the spring before day accumulations of mean air temperatures above C reach in warm-water streams, and before they reach 1050...