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Chemical techniques employed for the primary structural analysis of proteins and peptides

, : Chemical techniques employed for the primary structural analysis of proteins and peptides. Exs 73: 141-171

This chapter summarises modern microchemical approaches to the purification, identification and primary structure analysis of peptides and proteins. Discussion of high-sensitivity purification methods is restricted to two-dimensional polyacrylamide gel electrophoresis (2-DE) and microbore/capillary column reversed-phase high-performance liquid chromatography (RP-HPLC). Associated techniques are discussed, particularly with respect to analysis of the products with current automated amino acid sequencers and mass spectrometers.


PMID: 7579972

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