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Complex intracellular signal transduction regulates tissue plasminogen activator and plasminogen activator inhibitor type-1 synthesis in cultured human umbilical vein endothelium


, : Complex intracellular signal transduction regulates tissue plasminogen activator and plasminogen activator inhibitor type-1 synthesis in cultured human umbilical vein endothelium. Scandinavian Journal of Clinical & Laboratory Investigation 55(4): 323-330

Endothelial cells are central in fibrinolysis because of their high production of both activators (t-PA, uPA) and inhibitors (PAI-1). The t-PA and PAI-1 synthesis could be regulated by signals transduction at several cellular levels. The purpose of this in vitro study, on cultured endothelial cells, was to explore the receptor/second messenger regulation of the t-PA and PAI-1 synthesis. Quiescent confluent human umbilical vein endothelial cells, cultured in passage 1, were exposed to different test substances. Samples from the conditioned medium were collected after 16 and 24 h and analysed for t-PA and PAI-1 antigen. All data presented were related to the data from control dishes (= 100%), in the same experiment. The results from the present study (mean +- 95% confidence interval) demonstrated the following. (1) Forskolin, with a documented direct cAMP-inducing effect, decreased the basal PAI-1 production to 61 +- 15%, and Na-nitroprusside, with a documented cGMP-inducing effect, increased the basal PAI-1 production to 141 +- 38% without affecting the basal t-PA production. The surface receptor agonists isoprenaline or ephedrine, which indirectly affect adenylate cyclase, had no effect on t-PA or PAI-1 production. (2) Phorbolester (PMA), which directly activates proteinkinase C (PKC), increased the basal t-PA and PAI-1 production to 350 +-71%, and 163 +- 35% respectively. (3) Thrombin, but not endothelin-1 (ET-1) increased the basal t-PA and PAI-1 production to 195 +- 34% and 136 +- 18%, respectively, indicating an PKC-mediated thrombin effect. (4) Endotoxin increased both the basal t-PA and PAI-1 production to 130 +- 20% and 136 +- 30%, respectively. These data indicated that various stimuli involve different pathways e.g. cAMP, cGMP and PKC. Pharmacological interference with the regulation of t-PA and PAI-1 must take this complexity into account.

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Other references

Diers, B.W.; Skorupska, H.T.; Rao Arelli, A.P.; Cianzio, S.R., 1996: The genetic relationship among plant introductions with resistance to soybean cyst nematodes. Near-homogeneous populations of Heterodera glycines races were produced: race 1 was maintained on Essex, race 2 on Pickett 71, race 3 on Essex, race 5 on PI88788 and race 14 on a mixture of Forrest and PI90763. A set of 38 soyabean plant introduct...

Fattal, D.R.; Ben-Shaul, A., 1993: A molecular model for lipid-protein interaction in membranes: the role of hydrophobic mismatch. The interaction free energy between a hydrophobic, transmembrane, protein and the surrounding lipid environment is calculated based on a microscopic model for lipid organization. The protein is treated as a rigid hydrophobic solute of thickness dP...

Horst, H.J.; Klötzl, G.; Stromberg, U., 1979: Metabolism and binding in vitro of 5 alpha-androstane-3 beta, 17 beta-diol and of 5 alpha-androstane-3 alpha, 17 beta-diol in cell fractions of rat ventral prostate and liver. Rat ventral prostate and liver were investigated for the binding in vitro to particulate fractions and for the metabolism of 5 alpha-androstane-3 beta, 17 beta-diol. Comparative investigations were carried out on the metabolism of 5 alpha-androsta...

Mandilara, G.; Mavridou, A.; Lambiri, M.; Vatopoulos, A.; Rigas, F., 2006: The use of bacteriophages for monitoring the microbiological quality of sewage sludge. The use of bacteriophages as potential indicators of faecal pollution has recently been studied. The correlation of the number of bacterial indicators and the presence of three groups of bacteriophages, namely somatic coliphages (SOMCPH), F-RNA sp...

Metzgar, R.S.; Rodriguez, N.; Finn, O.J.; Lan, M.S.; Daasch, V.N.; Fernsten, P.D.; Meyers, W.C.; Sindelar, W.F.; Sandler, R.S.; Seigler, H.F., 1984: Detection of a pancreatic cancer-associated antigen (DU-PAN-2 antigen) in serum and ascites of patients with adenocarcinoma. A competition radioimmunoassay was developed, utilizing a murine monoclonal antibody to human pancreatic adenocarcinoma cells. Immunoblotting of a standard antigen preparation from either serum or ascites fluid after electrophoresis in 1% agarose...

Ratnikova, L.A.; Chistiakov, V.V.; Iaguzhinskiĭ, L.S., 1978: Regulatory interaction between the respiratory chain of mitochondria and the oxidative system of the endoplasmic reticulum. A regulation mechanism of the interaction of microsomal oxidases and mitochondrial respiratory chain with oxidase substrates is suggested. Quantitative comparison of their affinity to microsomal oxidases and mitochondrial NADH-dehydrogenase is car...

Bogen, K.T.; Singhal, A., 2016: Malathion dermal permeability in relation to dermal load: Assessment by physiologically based pharmacokinetic modeling of in vivo human data. NlmCategory="UNASSIGNED">Estimates of dermal permeability (K<sub>p</sub>), obtained by fitting an updated human PBPK model for malathion to previously reported data on excreted urinary metabolites after 29 volunteers were...

Moreno Romo M.A.; Fernandez G.S., 1986: Mycoflora of commercial poultry mixed feeds. Mycological analysis of 125 samples of commercial poultry mixed feeds were performed. Penicillium, Aspergillus, and yeast were present in more than 80% of the samples; Fusarium, Mucor, Cladosporium, and Aureobasidium were in about 50%, of the samp...

Sergeev P.V., 1974: The effect of aminazine and tisercin on the tissue blood barrier of the skin in inflammation. Farmakologiya I Toksikologiya (moscow): 570-573

Pravdina, N.F.; Galegov, G.A., 1970: The role of uridine in the synthesis of phage specific DNA in the cells of Escherichia coli infected by T2 phage. Biokhimiia 35(1): 85-88