geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Contribution of hydroxymethylbutenyl diphosphate synthase to carotenoid biosynthesis in bacteria and plants


, : Contribution of hydroxymethylbutenyl diphosphate synthase to carotenoid biosynthesis in bacteria and plants. Biochemical and Biophysical Research Communications 371(3): 510-514

The methylerythritol 4-phosphate (MEP) pathway synthesizes the precursors of carotenoids and other isoprenoids in bacteria and plant plastids. Despite recent progress in the identification of rate-determining steps, the relative contribution of most pathway enzymes to flux control remains to be established. In this work we investigated whether upregulated levels of hydroxymethylbutenyl diphosphate synthase (HDS) could increase the metabolic flux through this pathway, as judged by endpoint (carotenoid) measurements. Unlike other MEP pathway enzymes, however, increasing the levels of an active HDS protein in carotenoid-producing Escherichia coli cells and transgenic Arabidopsis thaliana plants did not result in an enhanced accumulation of MEP-derived isoprenoids. Our data suggest that enhanced flux through the MEP pathway for peak demand periods in bacteria and plastids does not require increased HDS activity.

US$19.90

PMID: 18452711

DOI: 10.1016/j.bbrc.2008.04.115


Other references

Rehman, M.Ur.; Narchi, H., 2015: Metabolic bone disease in the preterm infant: Current state and future directions. Neonatal osteopenia is an important area of interest for neonatologists due to continuing increased survival of preterm infants. It can occur in high-risk infants such as preterm infants, infants on long-term diuretics or corticosteroids, and thos...

Tamsma, A.; Bell, R.W., 1957: A new dairy product for ice cream. Sweetened modified cream. A new dairy product with high fat content and preserved with sugar may be prepared simply and economically. Cream with high fat content has nonfat dry milk added to stabilize the emulsion, sugar added to preserve and the mixture pasteurized. The c...

Miyabe, K.; Sotoura, S.; Guiochon, G., 2001: Retention and mass transfer characteristics in reversed-phase liquid chromatography using a tetrahydrofuran-water solution as the mobile phase. The characteristics of the retention and the mass transfer kinetics in reversed-phase liquid chromatography (RPLC) were measured on a system consisting of a C18-silica gel and a tetrahydrofuran-water (50:50, v/v) solution. These parameters were de...

Dolle, R.; Nultsch, W., 1988: Specific binding of the calcium channel blocker tritiated verapamil to membrane fractions of chlamydomonas reinhardtii. Specific binding of the calcium antagonist [3H]verapamil to a microsomal fraction, a presumptive plasma membrane fraction and an intracellular membrane fraction of the phototactic unicellular green alga Chlamydomonas reinhardtii has been demonstra...

Matthews John F.Jr., 1964: Los Medanos gas field. This is a brief summary of the areal geology, history, and production of the Los Medanos gas field, Contra Costa County, Calif.

Darbellay, C., 1983: What agriculture expects from tourism. Erwerbskombinationen in der Berglandwirtschaft: Thema Hrsg B Walther und HW Popp: 164

Herbert, R.D.; Gandevia, S.C.; Allen, G.M., 1997: Sensitivity of twitch interpolation. Muscle & Nerve 20(4): 521-523

Matyka, S.; Jaskiewicz, T.; Korol, W., 1996: Activity of multienzyme feed preparations during the thermal process used in the production of feed mixtures. The enzyme preparations L (consisting of cellulose, beta -glucanase, amylase and protease) and J (made up of cellulase, protease, beta -glucanase and xylanase) were added to barley at 0.5 and 0.1 g/kg, respectively, during autoclaving or extrusion...

van der Molen, A.B.; Birndorf, M.; Dzwierzynski, W.W.; Sanger, J.R., 1999: Subcutaneous tissue emphysema of the hand secondary to noninfectious etiology: a report of two cases. Subcutaneous emphysema of the hand can be benign and noninfectious in origin. Emphysema from gas-forming organisms is associated with systemic symptoms, whereas benign subcutaneous emphysema is not. High-pressure pneumatic tool injuries are a well...

Horii, Y.; Nagai, K.; Nakashima, T., 2013: Order of exposure to pleasant and unpleasant odors affects autonomic nervous system response. When mammals are exposed to an odor, that odor is expected to elicit a physiological response in the autonomic nervous system. An unpleasant aversive odor causes non-invasive stress, while a pleasant odor promotes healing and relaxation in mammals...