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Cooperative control via lymphoid enhancer factor 1/T cell factor 3 and estrogen receptor-alpha for uterine gene regulation by estrogen


, : Cooperative control via lymphoid enhancer factor 1/T cell factor 3 and estrogen receptor-alpha for uterine gene regulation by estrogen. Molecular Endocrinology 22(5): 1125-1140

Accumulating evidence indicates that estrogen regulates diverse but interdependent signaling pathways via estrogen receptor ( ER)-dependent and - independent mechanisms. However, molecular relationship between these pathways for gene regulation under the direction of estrogen remains unknown. To address this possibility, our uterine analysis of Wnt/beta-catenin downstream effectors revealed that lymphoid enhancer factor 1 ( Lef-1) and T cell factor 3 ( Tcf-3) are up-regulated temporally by 17 beta-estradiol (E-2) in an ER-independent manner. Lef-1 is abundantly up-regulated early ( within 2 h), whereas Tcf-3 is predominantly induced after 6 h, and both are sustained through 24 h. Interestingly, activated Lef-1/ Tcf-3 molecularly interacted with ER alpha in a time-dependent manner, suggesting they possess a cross talk in the uterus by E-2. Moreover, dual immunofluorescence studies confirm their colocalization in uterine epithelial cells after E-2. Most importantly, using chromatin immunoprecipitation followed by PCR analyses, we provide evidence for an interesting possibility that ER alpha and Tcf-3/Lef-1 complex occupies at certain DNA regions of estrogen-responsive endogenous gene promoters in the mouse uterus. By selective perturbation of activated Lef1/ Tcf-3 or ER alpha signaling events, we provide in this study novel evidence that cooperative interactions, by these two different classes of transcription factors at the level of chromatin, direct uterine regulation of estrogen-responsive genes. Collectively, these studies support a mechanism that integration of a nonclassically induced beta-catenin/Lef-1/ Tcf-3 signaling with ER alpha is necessary for estrogen-dependent endogenous gene regulation in uterine biology.

US$19.90

PMID: 18202148

DOI: 10.1210/me.2007-0445


Other references

Abom, W.A.; Keller, K.J., 1994: Characterization and desensitization of excitatory amino acid stimulated dopamine release in rat olfactory tubercle slices. Society for Neuroscience Abstracts 20(1-2): 731

Kumar, A.; Miller, D.; Ewbank, D.; Yousem, D.; Newberg, A.; Samuels, S.; Cowell, P.; Gottlieb, G., 1997: Quantitative anatomic measures and comorbid medical illness in late-life major depression. The authors examined the individual and relative roles of atrophy, comorbid medical illness, and cerebrovascular risk factors in the pathogenesis of late-life major depressive disorder (MDD). They used magnetic resonance imaging techniques to stud...

Parker, Aj, 1980: Treatment of feline and canine seizure disorders. Current veterinary therapy small animal practice(7): 830-837

Pitts, W.Reid., 2008: Predicting outcome in minimally invasive (T1a and T1b) urothelial bladder cancer using a panel of biomarkers: a high-throughput tissue microarray analysis. Bju International 101(12): 1595-1595

Baudet, J., 2000: Bone reconstruction. Synthesis of procedures and indications. Annales de Chirurgie Plastique et Esthetique 45(3): 405-408

Jiang, L.; Chen, X.; Lu, N.; Chi, L., 2014: Spatially confined assembly of nanoparticles. The ability to assemble NPs into ordered structures that are expected to yield collective physical or chemical properties has afforded new and exciting opportunities in the field of nanotechnology. Among the various configurations of nanoparticle...

Antalíková, R.; Hansen, T.G.B.; de la Mata, M.L.; Martínez, R., 2017: Investigating interdependent self in post-communist countries: A comparison of two Slovak and Danish generations. Some evidence suggests prevalence of collectivist values and interdependent self in post-communist Europe. However, research on social representations identifies a possible divide between Eastern Europeans' appreciation of their immediate soc...

D.J.sus, D.C.nha; Abreu, A.D.mingos, 1998: Contribution to the knowledge of the soft bottom echinoderms of Madeira Island. This paper paper presents a list of echinoderm species collected during a study devoted to the soft bottom macroinvertebrates of Madeira Island. One hundred and twenty stations were established between 20 and 100 meter's depth along the south...

Jarvis P.G.; Sandford A.P., 1986: The measurement of carbon dioxide in air. Marshall, B And F I Woodward (Ed ) Society For Experimental Biology Seminar Series, 22 Instrumentation For Environmental Physiology; Annual Conference Of The Environmental Physiology Group (Society For Experimental Biology) Held Jointly With The Association Of Applied Biologists And The British Ecological Society, Apr 6-8, 1983 Xi+242p Cambridge University Press: New York, N Y , Usa; Cambridge, England Illus 29-58, 1985

Saragusty, J.; Arav, A., 2011: Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification. Preservation of female genetics is currently done primarily by means of oocyte and embryo cryopreservation. The field has seen much progress during its four-decade history, progress driven predominantly by research in humans, cows, and mice. Two b...