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Detailed glomerular ultrastructure in Japanese type 2 diabetic patients by the quick-freezing and deep-etching method


, : Detailed glomerular ultrastructure in Japanese type 2 diabetic patients by the quick-freezing and deep-etching method. Diabetes Research and Clinical Practice 80(3): 424-431

Mesangial expansion and glomerular basement membrane (GBM) thickening did not correlate with urinary albumin excretion (UAE) in type 2 diabetic patients in our previous studies; therefore, it was necessary to elucidate more detailed ultrastructural changes in the early stages of diabetic nephropathy (DN) in type 2 diabetic patients. The quick-freezing and deep-etching (QF-DE) method allows us to examine three-dimensional ultrastructures of human renal glomeruli in vivo at high resolution. The QF-DE method was applied to six type 2 diabetic patients without definable renal diseases other than DN. Four patients were normoalbuminuric (NA) and the other two were microalbuminuria (MA). Three control specimens were the normal parts from nephrectomies due to renal cell carcinomas. Electron microscopic morphometric analyses provided quantitative glomerular structural changes. Replica membranes were prepared by the QF-DE method, and diameters of mesh structures at the GBM and mesangial matrix (MM) were measured on electron micrographs as previously described. By the QF-DE method, both the GBM middle layer and MM were composed of polygonal meshwork structures. The mesh pores of the GBM and MM were more enlarged and irregular in shape in NA diabetic patients than those of the controls, and these ultrastructural changes became more obvious in MA patients. The mesh diameters of the GBM and MM in the diabetic patients were also larger than those of the controls. Such a mesh diameter of the GBM was well correlated with the amount of UAE, while the mesh diameter of MM showed a slight correlation with UAE. Although there were small number of subjects in the present study, the detailed ultrastructural changes in NA and MA type 2 diabetic patients, which had not been disclosed by conventional electron microscopy, were revealed by the QF-DE method. Increased mesh diameters of GBM might be related with the increase of UAE.

US$19.90

PMID: 18342978

DOI: 10.1016/j.diabres.2008.01.027


Other references

Zeligman, I., 1955: Bismuth arthralgia. A.M.A. Archives of Dermatology 71(1): 108-111

Sung, R.Y., 1979: Total nose reconstruction: a single stage method. Numerous methods are used to repair nasal defect, but they usually need several operative stages. A single stage method for total nose reconstruction is presented. Patients (9) with total loss of nose were treated by this method. Results as good a...

Mathivat, A.; Clement, D.; Pelissier, C., 1962: Atypical tracings of ventricular repolarization of undetermined origin: 73 cases. Archives des Maladies du Coeur et des Vaisseaux 55: 890-908

Merickse-Stern, R.; Aerni, D.; Geering, A.H.; Buser, D., 2001: Long-term evaluation of non-submerged hollow cylinder implants. Clinical and radiographic results. From 1974 various types of hollow cylinder ITI-implants were placed before the new generation of Bonefit ITI-implants was developed in the mid-eighties. The aim of this study was the clinical and radiographic evaluation of hollow cylinder implants...

Carta, M.; Fortunato, A.; Zamperetti, N.; Giavarina, D.; Piccinni, P.; Soffiati, G., 2001: Usefulness of a semi-quantitative immunoassay for rapid measurement of procalcitonin. Clinical Chemistry & Laboratory Medicine 39(Special Supplement): S331

Bristowe, W.S., 1929: The distribution and dispersal of Spiders. Proceedings of the Zoological Society of London, 633-657

Hossler, P.; Racicot, C.; Chumsae, C.; McDermott, S.; Cochran, K., 2016: Cell culture media supplementation of infrequently used sugars for the targeted shifting of protein glycosylation profiles. Mammalian cells in culture rely on sources of carbohydrates to supply the energy requirements for proliferation. In addition, carbohydrates provide a large source of the carbon supply for supporting various other metabolic activities, including th...

Coster, W.J.; Kramer, J.M.; Tian, F.; Dooley, M.; Liljenquist, K.; Kao, Y-Chia.; Ni, P., 2016: Evaluating the appropriateness of a new computer-administered measure of adaptive function for children and youth with autism spectrum disorders. The Pediatric Evaluation of Disability Inventory-Computer Adaptive Test is an alternative method for describing the adaptive function of children and youth with disabilities using a computer-administered assessment. This study evaluated the perfor...

Kim, M.; Cho, S.Yun.; Han, I.Soo.; Koh, S.Don.; Perrino, B.A., 2005: CaM kinase II and phospholamban contribute to caffeine-induced relaxation of murine gastric fundus smooth muscle. Caffeine has been shown to increase the Ca2+ release frequency (Ca2+ sparks) from the sarcoplasmic reticulum (SR) through ryanodine-sensitive stores and relax gastric fundus smooth muscle. Increased Ca2+ store refilling increases the frequency of...

Fagade, S.O., 1976: Evaluation of chemical and mechanical weed control methods in upland rice culture. In field trials with upland rice cv. OS6, weeds were controlled by hand-hoeing or by application of propanil at 3-6 kg/ha or by combined mechanical and chemical means. Of the weed species recorded, Acanthospermum hispidum and Ipomoea sp. were resi...