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Different types of steroids inhibit [3H]diprenorphine binding in mouse brain membranes


, : Different types of steroids inhibit [3H]diprenorphine binding in mouse brain membranes. General Pharmacology 31(5): 747-751

1. The binding of 60 drugs, mainly steroids, to opioid receptors was studied in crude membrane fractions from mouse brains. 2. Competition assays with the different drugs (5 x 10(-7)-10(-4)M) were performed by labeling opiate receptors with [3H]diprenorphine (0.3-0.4 nM). 3. Only 7 drugs (alpha,5beta-tetrahydrodeoxycorticosterone, megestrol acetate, mifepristone, 17alpha-ethynylestradiol, diethylstilbestrol, clomiphene citrate and tamoxifen citrate) inhibited [3H]diprenorphine binding more than 50% at the highest concentration assayed (10(-4) M). The IC50 values ranged between 6x10(-5) M. 4. Thus, the present results show that only a limited number of steroids, from diverse classes, bind to opiate receptors.

US$19.90

PMID: 9809473

DOI: 10.1016/s0306-3623(98)00110-4


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Manubens, A.; Canessa, P.; Folch, C.; Avila, M.; Salas, L.; Vicuña, R., 2007: Manganese affects the production of laccase in the basidiomycete Ceriporiopsis subvermispora. The authors have previously identified and characterized lcs, a gene encoding laccase in the white-rot basidiomycete Ceriporiopsis subvermispora. In this work, the effect of Mn2+ in the production of extracellular laccase in liquid cultures of thi...

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Maier, W., 1961: Cryptorchism and testicular retention. Monatsschrift für Kinderheilkunde 109: 149-151

Breilh, D.; Pellegrin, J.L.; Mercier, P.; Neau, D.; Malbec, I.; Frappier, S.; Dupon, M.; Ragnaud, J.M.; Saux, M.C., 1998: Population pharmacokinetic of saquinavir and ritonavir combination in 35 AIDS patients. Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 38: 20

Rudenko, I.S., 1978: Distant hybridization and polyploidy in fruit crops. This book contains the results of cytogenetic and morphological studies on interspecific and intergeneric hybrids and polyploids. F2 hybrids of the 2x, 3x and 4x type were obtained from quince X apple and apple X pear crosses. As a result of work...

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Bolot, F.; Tichadou, M., 1956: Carotid-jugular arteriovenous aneurysm; early surgery; cure. Maroc Medical 35(368): 49-50

Malik, S.S.; Nair, P.G., 1967: Studies on serological detection of cow-milk added to buffalo-milk. Cows' skim-milk and solutions of acid casein, Ham-mersten's soluble casein, sodium caseinate and dried skim-milk were injected into rabbits. Cross-reacting antibodies were absorbed from the sera with buffaloes' milk casein. The resu...

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