+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Elastase assays

, : Elastase assays. Methods in Enzymology 235: 554-562

Two methods of P. aeruginosa elastase purification are described: Method 1 involves concentration of sample supernatants, followed by DEAE-Sepharose liquid chromatography, whereas Method 2 involves initial fractionations followed by molecular sieving and hydrophobic interaction high-performance liquid chromatography. The choice of methods depends on the available equipment and supplies. The methods of assaying elastase activity described as useful for a variety of applications. The elastin-nutrient agar plate method is a qualitative assay to determine the presence of elastase activity produced by a given culture or colony. Use of the quantitative elastin-Congo red assay is appropriate for determining elastase activities of mid-to-high elastase-producing cultures. For more sensitive determinations of P. aeruginosa elastase activity, use of the fluorogenic substrate is advisable.


PMID: 8057926

Other references

Watt G.; Alquiza L.M.; Padre L.P.; Tuazon M.L.; Laughlin L.W., 1988: The rapid diagnosis of leptospirosis a prospective comparison of the dot elisa and the genus specific microscopic agglutination test at different stages of illness. Journal of Infectious Diseases 157(4): 840-842

Brain, C.K., 1976: A reinterpretation of the swartkrans site and its remains. A re-interpretation of the stratigraphy and stages in formation of the Swartkrans site was necessitated by geological and paleontological evidence. The Outer Cave, source of virtually all the fossils, is now thought to contain deposits of 3 distin...

Uozumi, H.; Baba, Y.; Yasunaga, T.; Takahashi, M., 1987: Experimental and clinical evaluation of a prototype hyperthermia system. In order to achieve better thermal distribution, a high output radiofrequency capacitive heating system was developed for hyperthermia and operated at a frequency of 13.56 MHz with an output of 1,000 watts (Inter Nova Inc., IH-1000). With use of t...

Anonymous, 1990: Cyclical etidronate treatment of osteoporosis. New England Journal of Medicine 323(23): 1633-1635

Meregalli, M.; Osella, G.; Zuppa, A.M.ria, 2006: The Raymondionymidae of the Curti collection, with description of Raymondionymus curtii sp. n. (Coleoptera, Curculionoidea). The Coleoptera Curculionoidea Raymondionymidae collected by Marc Curti and preserved in the Museum d'histoire naturelle, Geneva, Switzerland were studied. A list of the 16 species and all specimens is given. Taxonomy, mutual relationships and...

Bowman V., 1998: High palaeolatitude forests from the Middle Jurassic of New Zealand. Pages 12 1998

Erfle, H.; Simpson, J.C.; Bastiaens, P.I.H.; Pepperkok, R., 2004: siRNA cell arrays for high-content screening microscopy. RNA interference (RNAi) is a recent advance that provides the possibility to reduce the expression of specific target genes in cultured mammalian cells with potential applications on a genome-wide scale. However, to achieve this, robust methodolog...

Gouyon, J.B.; Debbas, N.; Allaert, F.A.; Hakim, M.; Alison, M.; Nivelon, J.L.; Dusserre, L., 1985: Do intrauterine growth retardation and low ponderal index predict various aspects of neonatal morbidity?. Presse Medicale 14(28): 1518-1518

Hoste,R., 2008: Piglet exports to Germany a market to be won; action items for the Dutch pig sector. This study examines the opportunities for improvement of piglet sales from the Netherlands to Germany. Communication and even more attention devoted to the health of the piglets are important action items for the Dutch pig sector.

Bennett, R.L., 2003: Leading Voices and the Power of One: 2002 Presidential Address to the National Society of Genetic Counselors. Presented as the annual education conference of the National Society of Genetic Counselors in Phoenix, Arizona on November 11, 2002.