geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Fast hydrophilic interaction liquid chromatographic separations on bonded zwitterionic stationary phase


, : Fast hydrophilic interaction liquid chromatographic separations on bonded zwitterionic stationary phase. Journal of Separation Science 31(9): 1529-1536

Separation science is an art of obtaining adequate resolution of the desired compounds in minimum time, and with minimum effort in terms of sample preparation and data evaluation. In LC, where selectivity is a main driving force for separation, the availability of different separation modes capable of operating at high flow rates is a way to make combined optimal use of selectivity, efficiency, and speed. The separation of polar and hydrophilic compounds is problematic in RP LC due to the poor retention. Hydrophilic interaction liquid chromatography (HILIC) is a more straightforward separation mode to address this problem. Herein, it is shown that separations in HILIC mode are equally efficient as for RP, providing a potential for very fast separations on short columns. This is not only facilitated by the low viscosity of the mobile phase compositions used, compared to typical RP eluents, but also due to higher column permeability. To exemplify this, baseline separations of uracil and cytosine are shown in less than 4 s and of Tamiflu (R) and its main metabolite in less than 40 s, both under isocratic conditions. HILIC must therefore be considered having potential for high throughput purposes, and being an attractive candidate as the second separation dimension in 2-D HPLC.

US$19.90

PMID: 18461573

DOI: 10.1002/jssc.200800080


Other references

Marik Z., 1986: Sewage containing radioactive pollution. Vodni Hospodarstvi, Rada B 36(5): 117-120

Chan, J.K.; Fletcher, C.D.; Hicklin, G.A.; Rosai, J., 1990: Glomeruloid hemangioma. A distinctive cutaneous lesion of multicentric Castleman's disease associated with POEMS syndrome. A histologically distinctive cutaneous hemangioma occurring in two patients with biopsy-proven multicentric Castleman's disease associated with POEMS (polyneuropathy, organomegaly, endocrinopathy, M-protein, skin changes) syndrome are reporte...

Leisure, G.S.; O'Flaherty, J.; Green, L.; Jones, D.R., 1996: Propofol and postoperative pancreatitis. Anesthesiology 84(1): 224-227

Popovic, M.E.cegan, M.Z.kula, S., 1970: Effect of application of additives-stimulators in the rations of swine on the occurrence and resistance of some species of intestinal microflora. iI. Veterinarski glasnik: 4 (4) 261-266

Golovan' , V.T., 1980: The effect of udder stimulation in heifers on udder shape and function. Data were obtained on Red Steppe heifers (20 per group), beginning with the 6th mth of pregnancy. Heifers from the 1st group were given an udder massage twice daily, those from the 2nd group were subjected to simulated milking, and those from the...

Longo, F.M.; Massa, S.M., 2005: Neurotrophin-based strategies for neuroprotection. Neurotrophins activate a number of signaling pathways relevant to neuroprotection; however, their poor pharmacological properties and their pleiotropic effects resulting from interaction with the p75(NTR)-Trk-sortilin three-receptor signaling syst...

Hassel, J.C.; Danner, D.; Hassel, A.J., 2012: Psychosomatic or allergic symptoms? High levels for somatization in patients with drug intolerance. Patients with dermatological diseases often have associated psychological problems. For patients with allergic diseases, only a few publications have focused on psychological aspects. The objective of this study was to assess the psychometric prof...

Kramer J.; Feuerstein G.; Ben David M., 1979: The effect of angiotensin ii on in vitro prolactin and luteinizing hormone release from the rat pituitary. The effect of angiotensin II (A II) on prolactin (PRL) and LH [luteinizing hormone] release was studied in vitro using the rat pituitary hypothalamus system. Angiotensin induced a rapid, shortlasting, increase in PRL release followed by a 2nd peak...

Lax, I.P., 1983: Mens sana in corpore sano. Enfoque 11(1): 3-3

Topo, P.; Hemminki, E., 1995: Is menopause withering away?. Menopause (cessation of menstruation) and the period surrounding it (climacterium) are often defined retrospectively by asking a woman the date of her last menstrual period (LMP). Based on a survey of 2000 women aged 45-64 in 1989 in Finland, this...