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Lys Tag: an easy and robust chemical modification for improved de novo sequencing with a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer


, : Lys Tag: an easy and robust chemical modification for improved de novo sequencing with a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer. Rapid Communications in Mass Spectrometry 22(12): 1823-1833

Mass spectrometry using a matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) instrument is a widespread technique for various types of proteomic analysis. Along with an expanding interest in proteomics, there is a strong requirement for the identification of proteins with high confidence from biological samples. Peptide modification by a wide variety of posttranslational modifications (PTMs), the existence of different protein isoforms and the presence of uncharacterized genomes of many species, make protein identification through peptide mass fingerprinting (PMF) often unachievable. Peptide de novo sequencing has been proven to be a useful approach to overcome these variables, and efficient derivatization processes are important tools to achieve this goal. In the present work we describe the methodology and experimental applications of a fast, efficient and cheap lysine derivatization. This chemical modification improves the signals from lysine-terminated peptides and can be efficiently used as a lysine-blocking agent in combination with other derivatization techniques. Most importantly, upon peptide fragmentation it generates a neat series of predominantly y-ions, allowing the determination of unambiguous amino acid sequences. Moreover, this chemical compound was used with target-eluted samples, enabling a second, alternative analysis of the same sample in the MALDI mass spectrometer. Copyright (C) 2008 John Wiley & Sons, Ltd.

US$19.90

PMID: 18470875

DOI: 10.1002/rcm.3555


Other references

Kan, R.K.; Morin, D.S.; Shaubach, L.A.; Finger, J.N.; Mitchell, R.B.; Day, J.R.; Anthony, A., 1996: Basal forebrain RNA changes in relation to tau protein in Alzheimers disease. Society for Neuroscience Abstracts 22(1-3): 1421

Mizuno, K., 1968: Denaturation of rhodopsin caused by monochromatic light irradiation. Nihon Ganka Kiyo 19(1): 57-61

MacVane, C.Z.; Irish, C.B.; Strout, T.D.; Owens, W.B., 2013: Implementation of transvaginal ultrasound in an emergency department residency program: an analysis of resident interpretation. Emergency physicians are increasingly performing transvaginal ultrasound (TVUS) to rule out ectopic pregnancy. However, little is known about appropriate educational pathways to train emergency medicine residents in TVUS. To evaluate the ability o...

Bateman, T., 1818: Notes of a case of Mercurial Erethism. Medico-Chirurgical Transactions 9(Pt 1): 220-233

Kempski M.H.; Blackburn J.L.; H.N.; Clark E.B., 1992: Power spectrum analysis of dorsal aortic blood velocity in the stage 21 24 and 27 chick embryo. Pediatric Research 31(4 PART 2): 20A

Salimeh, A.; Mohammadi, M.; Rashidi, B., 2013: Preconditioning with diosgenin and treadmill exercise preserves the cardiac toxicity of isoproterenol in rats. This study was aimed to evaluate the preventive effect of diosgenin and exercise on tissue antioxidant status in isoproterenol-induced myocardial infarction (MI) in male Wistar rats. Levels of lipid peroxides, reduced glutathione (GSH), and the ac...

Hays, Sm, 1995: Joint venture pays ag dividends. Agricultural research 43(9): 4-10

Demarcq G., 1986: Geodynamic and paleogeographic relationships between the rhone provence france and the tyrrhenian italy areas in the miocene. Giornale di Geologia (Bologna) 48(1-2): 77-84

Pausch Gerhard; Bossew Peter; Hofmann Werner; Steger Ferdinand, 1998: Multifractal analysis of the (super 137) Cs fallout pattern in Austria resulting from the Chernobyl accident. Health Physics 74(6): 673-676

Dzido, T.H.; Lopaciuk, E.; Płocharz, Pł.W.; Chomicki, A.; Zembrzycka, M.; Frank, H., 2014: Equipment and preliminary results for orthogonal pressurized planar electrochromatography. We report combination of overpressured layer chromatography (OPLC) and pressurized planar electrochromatography (PPEC) techniques into a single technique in which both OPLC and PPEC processes proceed simultaneously and orthogonally. The separation...