+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Optimization of enzymatic methanolysis of soybean oil by response surface methodology

, : Optimization of enzymatic methanolysis of soybean oil by response surface methodology. Journal of the American Oil Chemists' Society 83(11): 929-932

Enzymatic methanolysis of refined soybean oil with methanol was investigated using Rhizomucor miehei lipase, Lipozyme RM IM, in n-hexane for reaction times of 30 min. Response surface methodology (RSM) based on three-level, three-factor (variable) face-centered cube design was used for the optimization of methanolysis. The independent variables that affect the methanolysis reaction conducted in n-hexane are temperature (degrees C), enzyme/oil weight ratio, and oil/methanol molar ratio. A good quadratic model was obtained for the methyl ester production by multiple regression and backward elimination. A linear relationship was observed between the observed and predicted values (R-2 = 0.9635). The effects of temperature and enzyme amount, which affected methyl ester content of the product (response) positively, were significant (P < 0.01). The quadratic term of temperature and the interaction term of enzyme amount with temperature affected the response negatively (P < 0.01). The interaction term of conditions for the response at which methyl ester content of the product was 76.9% were determined to be 50 degrees C, 2.37 methanol/oil mole ratio, and 0.09 enzyme/oil weight ratio.


DOI: 10.1007/s11746-006-5048-4

Other references

Opitz, B., 2010: Context-dependent repetition effects on recognition memory. One widely acknowledged way to improve our memory performance is to repeatedly study the to be learned material. One aspect that has received little attention in past research regards the context sensitivity of this repetition effect, that is whet...

Mcveigh, Ilda, 1936: Apical growth of the leaves of Camptosorus rhizophyllus. Each leaf originates from a superficial cell of the stem apex. By the first few divisions of this cell a "two-sided" apical cell is formed, bounded on the free surface by a convex lens-shaped surface and on the cutting faces by convex su...

Neuser D.; Hirth C.; Kazda S., 1989: The effect of calcium antagonists and calcium agonists on parathyroid hormone in rat acute renal failure. Naunyn-Schmiedeberg's Archives of Pharmacology 339(SUPPL): R44

Eschwege, E.; Swynghedauw, B.; Tran, M.H.; Claude, J.R.; Richard, J.L., 1970: The intravenous glucose load test in normal subjects and others with high triglyceride values. Variations in serum tri-glycerides. Men without known disorder were 20 controls with triglycerides under 180 mg per 100 ml plasma fasting and 27 others with over 180 mg. Fasting, they were given glucose loads of 0.33 g per kg bodyweight by vein. On average an index derived from body...

Bonchev, N.; Sandev, S., 1965: Amino acid composition of meat and milk after normal heat treatment. No significant change was found by paper chromatography in the amino acid composition of pasteurized milk from cows after it had been boiled for 30 min.

Inoue, M.; Maeno, T.; Miki, D.; Embabi, S.; Hatchell, D.L., 2004: Survival of subretinal pancreatic islet cell allografts and apoptosis in infiltrating lymphocytes in rats. Survival of pancreatic islet cells transplanted into the subretinal space in rat eyes was studied in relation with apoptosis of infiltrating lymphocytes. Islets isolated from inbred DA rats were transplanted into the subretinal space of fully MHC-...

D.S.yza N.; Kane J.; Bissett J.; Murphy M., 1974: Heart rate and its relationship to ventricular arrhythmia in acute myo cardial infarction in man. Circulation Supplement 50(4-3): III120

Sugiyama, M., 1950: Studies on the artificial parthenogenesis in the sea-urchin eggs. XVII. The conditions to induce the membrane formation by means of saponin. Zoological Magazine Tokyo, 59: 132-134

Sagie, A.; Larson, M.G.; Levy, D., 1993: The natural history of borderline isolated systolic hypertension. Background. Patients with isolated systolic hypertension are at increased risk for cardiovascular disorders. We attempted to determine whether those with borderline isolated systolic hypertension (defined as a systolic blood pressure of 140 to 159...

Nancib, A.; Nancib, N.; Meziane-Cherif, D.; Boubendir, A.; Fick, M.; Boudrant, J., 2004: Joint effect of nitrogen sources and B vitamin supplementation of date juice on lactic acid production by Lactobacillus casei subsp. rhamnosus. The use of date juice as a substrate for lactic acid production was investigated. Various nitrogen sources were compared with yeast extract for efficient lactic acid production by Lactobacillus casei subsp. rhamnosus. Among different nitrogen sour...