+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Production of a recombinant HA1 protein of swine influenza virus H5 subtype and development of an indirect ELISA

, : Production of a recombinant HA1 protein of swine influenza virus H5 subtype and development of an indirect ELISA. Chinese Veterinary Science: 9, 762-766

The HA1 gene of swine influenzavirus (SIV) H5 subtype was synthesized and subcloned into pET-28a(+) vector to form recombinant plasmid pET28a-HA1/H5. After being transformed with the recombinant plasmid, <i>Escherichia coli</i> BL21 (DE3) was induced by IPTG and the expressed protein was purified by Ni-chelated chromatography.


Other references

Goldberg, B.; Pecora, C., 1994: Stress Fractures. In brief We studied 3 years of stress fractures that occurred at a major university. We found an annual Incidence of 1.9% among athletes, but 67% of the injuries were in freshmen. In 86% of the cases, patients had abruptly changed their training-s...

Demay Andre, 1937: Sur l'age du metamorphisme dans le Massif Central. Hercynian age of the metamorphism in the Central Massif, France.

Miranda, C., 1944: Nota sobre os transmissores da molestia de Chagas ocorrentes no Estado no Maranhao, Brasil. Memorias do Instituto Oswaldo Cruz, 39(3): 297-300

Wierup, N.; Björkqvist, M.; Kuhar, M.J.; Mulder, H.; Sundler, F., 2006: CART regulates islet hormone secretion and is expressed in the beta-cells of type 2 diabetic rats. Cocaine- and amphetamine-regulated transcript (CART) is an anorexigenic peptide widely expressed in the central, peripheral, and enteric nervous systems. CART is also expressed in endocrine cells, including beta-cells during rat development and de...

Mansilla, J.P.; Iglesias, C.; Mazoy, D., 1993: Altica quercetorum Foudr. (Cole. Chrysomelidae, Alticinae), pest of oak in Galicia. The presence of Altica quercetorum in Galicia, Spain, is reported. This species causes serious defoliation of Quercus robur. Distribution, life cycle and control are described.

Erkut, S.; Eminkahyagil, N.; Imirzalioglu, P.; Tunga, U., 2004: A technique for restoring an overflared root canal in an anterior tooth. This article describes a technique for fabricating a dowel and core for a tooth with an overflared root canal. The technique involves supporting a glass fiber-reinforced prefabricated dowel with chopped silanated glass fibers.

Rouchaud, J.; Gustin, F.; Cappelen, O.; Mouraux, D., 1994: Pig slurry and cow manure effect on atrazine and metolachlor soil biodegradation in maize. Bulletin of Environmental Contamination and Toxicology 52(4): 568-573

Vakh A.S.; Sapin V.I., 1984: New discovery of iordanite in auro-polymetallic ores. Doklady Akademii Nauk SSSR 276(2): 446-450

Chen, P.; Vicini, F.; Kestin, L.; Wallace, M.; Mitchell, C.; Benitez, P.; Pettinga, J.; Martinez, A., 2003: Long-term cosmetic results and toxicity with accelerated partial breast irradiation utilizing interstitial brachytherapy. International Journal of Radiation Oncology Biology Physics 57(2 Supplement): S309-S310

Serga, V.I.; Kaysh, E.N., 1967: On injuries of the chest during peace time. Klinicheskaia Khirurgiia 5: 9-11