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Production of a recombinant HA1 protein of swine influenza virus H5 subtype and development of an indirect ELISA


, : Production of a recombinant HA1 protein of swine influenza virus H5 subtype and development of an indirect ELISA. Chinese Veterinary Science: 9, 762-766

The HA1 gene of swine influenzavirus (SIV) H5 subtype was synthesized and subcloned into pET-28a(+) vector to form recombinant plasmid pET28a-HA1/H5. After being transformed with the recombinant plasmid, <i>Escherichia coli</i> BL21 (DE3) was induced by IPTG and the expressed protein was purified by Ni-chelated chromatography.

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