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Protective effects of vanadium against DMH-induced genotoxicity and carcinogenesis in rat colon: removal of O(6)-methylguanine DNA adducts, p53 expression, inducible nitric oxide synthase downregulation and apoptotic induction

, : Protective effects of vanadium against DMH-induced genotoxicity and carcinogenesis in rat colon: removal of O(6)-methylguanine DNA adducts, p53 expression, inducible nitric oxide synthase downregulation and apoptotic induction. Mutation Research 650(2): 123-131

Previous studies have shown that dietary micronutrient vanadium can protect neoplastic development induced by chemical carcinogens. Current investigation is an attempt to evaluate the role of vanadium (4.27 mu mol/l) in inhibiting 1,2 dimethyhydrazine (DMH) (20 mg/kg body weight) induced rat colon carcinogenesis. We investigated the effect of vanadium against the formation of DMH-induced O-6-methylguanine (O-6 -Meg) DNA adduct, a potent cytotoxic and mutagenic agent for colon cancer. Supplementation of vanadium significantly reduced the hepatic (P < 0.05), and colonic (at three sequential time points; ANOVA, F = 4.96, P < 0.05) O-6 -Meg DNA adduct levels in rats, indicating vanadium's potency in limiting the initiation event of colon carcinogenesis. Removal of initiated and damaged precancerous cells by apoptosis can prevent tumorigenesis and further malignancy. DNA fragmentation study revealed the vanadium-mediated apoptotic induction in colon tumors. The increased value of apoptotic index (AI) (62.27%; P < 0.0 1) in subsequent TUNEL assay further confirmed the apoptosis induction by vanadium. This paralleled the nuclear immunoexpression of p53. A significant positive correlation between p53 immunoexpression and AI (P = 0.0026, r = 0.83, r(2) = 0.69) links its association with vanadium-mediated apoptotic induction. Vanadium treatment also abated the mRNA expression of iNOS (54.03%), reflecting its protective effect against nitric oxide-mediated genotoxicity and colon tumorigenesis. These studies cumulatively provide strong evidence for the inhibitory actions of vanadium against DMH-induced genotoxicity and carcinogenesis in rat colon.


PMID: 18155637

DOI: 10.1016/j.mrgentox.2007.11.001

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Mcdougall, E.M.; Rabinowitz, R.; Luttman, D., 1996: Laparoscopic management of vaginal prolapse. Journal of Endourology 10(SUPPL 1): S97

Stump, C.S., 2012: Physical Activity in the Prevention of Chronic Kidney Disease. Chronic kidney disease (CKD) is an important risk factor for cardiovascular disease (CVD) and mortality. The increase in CKD in recent decades has paralleled increases in obesity, diabetes, and the metabolic syndrome. Physical inactivity is a modi...

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Nagle, R.E.; Williams, D.O., 1974: Natural history of ventricular aneurysm without surgical treatment. British Heart Journal 36(10): 1037-1037

Yamaguchi, H.; Nakazato, Y.; Shoji, M.; Okamoto, K.; Ihara, Y.; Morimatsu, M.; Hirai, S., 1991: Secondary deposition of beta amyloid within extracellular neurofibrillary tangles in Alzheimer-type dementia. The hippocampal areas of 34 autopsy specimen brains from aged demented and nondemented subjects were examined using double staining of Bodian and beta protein. In 18 cases (75.5 +/- 7.4 years old), none of the extracellular neurofibrillary tangles...

Gold P.W.; Goodwin F.K.; Chrousos G.P., 1988: Clinical and biochemical manifestations of depression relation to the neurobiology of stress second of two parts. New England Journal of Medicine 319(7): 413-420

Hilliard, N., J.; Johnson, C., N.; Armstrong, S., H.; Quarles, S.; Waites, K., B., 2002: In vitro activity of ertapenem (MK-0826) against multi-drug resistant Streptococcus pneumoniae compared with 13 other antimicrobials. We tested ertapenem (MK-0826), a new carbapenem, and 13 other antimicrobials by microbroth dilution against 102 isolates of Streptococcus pneumoniae, selected to include organisms resistant to a variety of drug classes. Ertapenem MICs ranged from...

Mowli, V.C., 1990: Of human bondage: an Indian experience amongst the agrarian workforce. The historical background to bonded labour is examined together with the legal structures for releasing the bonded and constraints to freedom. Types and incidence of bonded labour are first discussed, followed by case studies of the traditional sy...

Jardine, S.C.mbaz, A.M.gloire, L.P.niguel, G.V.chey, G., 1974: Stratigraphical distribution of acritarchs in the Palaeozoic of the Algerian Sahara. Review of palaeobotany and palynology: 18 (1 2) 99-129

Meshcheryakov, VN., 1978: Isolation of the mitotic apparatus from the eggs and embryos of pulmonate mollusc Lymnaea stagnalis L. The mitotic apparatus from zygotes and embryos of the pond snail L. stagnalis was isolated, using modified methods by Kane (1962), and Forer and Zimmerman (1976). The isolation medium contained Triton X-100. No preliminary removal of the vitelline...