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Regeneration of transgenic kale , rape and turnip plants via Agrobacterium rhizogenes mediated transformation


, : Regeneration of transgenic kale , rape and turnip plants via Agrobacterium rhizogenes mediated transformation. Plant Science (Limerick) 87(2): 161-169

Transgenic hairy root lines were developed for six Brassica cultivars: B. napus cv. Giant (rape), B. oleracea cvs. Kapeti, Midas, Rawara and Medium Stem (all kales) and B. campestris cv. Red Globe (turnip). Leaf explants or petioles of intact cotyledons were cocultivated with Agrobacterium rhizogenes strain AT harbouring the binary vector pKIW110, the T-DNA of which contains genes encoding beta-glucuronidase (GUS), neomycin phosphotransferase II (NPTII) and chlorsulfuron resistance. Hairy root lines grew prolifically on hormone-free medium containing selective levels of kanamycin (50 to 100 mg/l) or chlorsulfuron (10 mu-g/l). Transgenic shoots were regenerated from hairy roots of all cultivars and plants have been successfully transferred to soil. Molecular analyses using polymerase chain reaction (PCR) and Southern blotting confirmed the transgenic nature of these plants.

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Other references

Simmons, Hc, 1988: The role of industry in developing innovative spraying equipment. Improving on target placement of pesticides: a conference: June 13-15-1988 Sheraton International Conference Center Reston VA sponsored by the Agric Res Inst in cooperation with the USDA Science and Education: 4

Ozcelik, D.; Toplan, S.; Akyolcu, M.C., 2004: Effects of lithium on copper and zinc in the serum, liver, brain and kidney of rats. Introduction and purpose: Lithium is the treatment of choice in maniac depression psychiatry. In this study, we investigated the possible effects of lithium on the essential trace elements of copper and zinc in brain, liver and kidney of rats. Mat...

Bellisai, Iole, 1929: Researches on the photo-tactic reactions in Artemia salina. In Artemia salina univalens, phototactic reactions are the same in both sexes. While the larvae have only the median Nauplius eye, they are completely positive but the reaction becomes weaker and gradually negative as the compound eyes develop. Li...

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Vesell, E.S.; Philip, J.; Bearn, A.G., 1962: Comparative studies of the isozymes of lactic dehydrogenase in rabbit and man. Observations during development and in tissue culture. During development of rabbit tissues, characteristic sequential alterations in the LDH isozyme pattern occur, and consist for liver and muscle in loss of the most rapidly migrating anodal bands, and increased activity in the cathodal bands and slo...

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Yeang H.Y.; Hillman J.R., 1981: Control of lateral bud growth in phaseolus vulgaris cultivar canadian wonder by ethylene in the apical shoot. Elongation growth of the 1st trifoliate leaf axillary bud was induced by physical restriction of the apical growth or by treating the apical shoot with ethylene or ethephon. The promotion of axillary bud development by ethylene action on the apica...

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