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Renaturation of recombinant proteins produced as inclusion bodies


, : Renaturation of recombinant proteins produced as inclusion bodies. Biotechnology Advances 12(1): 89-101

Expression of recombinant proteins in Escherichia coli often results in the formation of insoluble inclusion bodies. Within the last few years specific methods and strategies have been developed to prepare active proteins from these inclusion bodies. These methods include (i) isolation of inclusion bodies after disintegration of cells by mechanical forces and purification by washing with detergent solutions or low concentrations of denaturant, (ii) solubilization of inclusion bodies with high concentrations of urea or guanidine-hydrochloride in combination with reducing reagents, and (iii) renaturation of the proteins including formation of native disulphide bonds. Renatured and native disulphide bond formation are accomplished by (a) either air oxidation, (b) glutathione reoxidation starting from reduced material, or (c) disulphide interchange starting from mixed disulphides containing peptides. The final yield of renatured proteins can be increased by adding low concentrations of denaturant during renaturation.

US$19.90

PMID: 14547821

DOI: 10.1016/0734-9750(94)90292-5


Other references

Rosenfeld, J.V.; Widaa, H.A.; Adams, C.B., 1991: Cerebral arteriovenous malformation causing benign intracranial hypertension--case report. A 32-year-old male with a large arteriovenous malformation (AVM) in the right temporoparietal area presented with features of benign intracranial hypertension. This association is rare. The pathogenesis is believed to be due to cerebral venous hyp...

Peloquin, S.M., 1994: Occupational therapy as art and science: should the older definition be reclaimed?. American Journal of Occupational Therapy 48(11): 1093-1096

Moya Maria Cristina, 1997: The Iruya unconformity in the Andes of northwestern Argentina; evidence of glaciation during the late Upper Cambrian?. Gaea Heidelbergensis 3: 242-243

Santa, T.; Kimoto, K.; Fukushima, T.; Homma, H.; Imai, K., 1996: 4-(N-hydrazinoformylmethyl-N-methyl)-amino-7-N, N-dimethylaminosulphonyl-2,1,3-benzoxadiazole (DBD-CO-Hz) as a precolumn fluorescence derivatization reagent for carboxylic acids in high-performance liquid chromatography. A new fluorescent reagent for carboxylic acids, 4-(N-hydrazinoformylmethyl-N-methyl)amino-7-N,N-dimethylaminosu lphonyl-2, 1,3-benzoxadiazole (DBD-CO-Hz) was synthesized and its applicability as a precolumn derivatization reagent in high-performan...

Appelbaum, A.L.; Grayson, M.A., 1977: AMA postpones debate on cost containment report. "Aggressive moderation" to combat government's excess was the position suggested by John H. Budd, M.D., in his presidential address to the AMA's House of Delegates. Topics under discussion at this interim meeting included cost...

Sineshchekov O.A.; Sudnitsyn V.V.; Litvin F.F., 1988: Periodic micromovements of the protoplast of the unicellular flagellate alga haematococcus pluvialis. Biophysics (English Translation of Biofizika) 33(2): 399-401

Chudek J.A.; Foster R.; Davison I.R.; Reed R.H., 1984: Altritol in the brown alga himanthalia elongata. Natural abundance 13C NMR spectroscopy showed that altritol (talitol) is a major constituent within vegetative and reproductive tissues of H. elongata. Quantitative GC [gas chromatography] studies confirmed that altritol concentrations are 80-160%...

Evans, D.G.; Nash, J.E., 2001: Transmyocardial revascularization system and method of use. A transmyocardial revascularization system including a plurality of inserts formed of a material to elicit a healing response in tissue of the myocardium and deployment instruments and associated components for deploying the inserts into the wall...

Lucas, J.S.; du Toit, G.; Lloyd, K.; Sinnott, L.; Forster, D.; Austin, M.; Clark, C.; Tuthill, D.; Brathwaite, N.; Warner, J., 2012: The RCPCH care pathway for children with latex allergies: an evidence- and consensus-based national approach. The Royal College of Paediatrics and Child Health (RCPCH) Science and Research Department was commissioned by the Department of Health to develop national care pathways for children with allergies; the latex allergy pathway is the seventh pathway....

van der Spek, P.J.; Eker, A.; Rademakers, S.; Visser, C.; Sugasawa, K.; Masutani, C.; Hanaoka, F.; Bootsma, D.; Hoeijmakers, J.H., 1996: XPC and human homologs of RAD23: intracellular localization and relationship to other nucleotide excision repair complexes. The xeroderma pigmentosum syndrome complementation group C (XP-C) is due to a defect in the global genome repair subpathway of nucleotide excision repair (NER). The XPC protein is complexed with HHR23B, one of the two human homologs of the yeast N...