+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Sequence comparison and mutational analysis of elements that may be involved in the regulation of DNA synthesis in HIV-1

, : Sequence comparison and mutational analysis of elements that may be involved in the regulation of DNA synthesis in HIV-1. Journal of Molecular Evolution 37(2): 198-203

The large number of sequenced clones of HIV-1 and related viruses made it possible to indicate conserved elements with potential regulatory or structural functions. Such analysis was combined with directed mutagenesis in order to investigate the importance of elements that may influence the initiation of plus-strand DNA synthesis. The main site for plus-strand initiation is a polypurine tract near the 3' end of the viral RNA (the 3' PPT). An exact copy of this PPT is located in the middle of the genome (the internal PPT). Upstream from the internal PPT there is an inverted repeat. Mutants designed to upset the internal PPT (i.e., purine to pyrimidine changes), as well as mutants designed to abolish the potential stem-loop formation (changes around the internal PPT or in the upstream inverted repeat) both resulted in viruses with a reduced ability to replicate. Upsetting the stem-loop formation was, however, less harmful than changing the polypurine nature of the PPT. Changing a conserved T on the 3' side of the PPT to a C did not affect the phenotype.


PMID: 8411209

DOI: 10.1007/bf02407356

Other references

Shevera M.V., 1989: Critical taxa of the genus chamaecytisus link in the ukrainian flora ussr. As a result of the complex biosystematic research and critical analysis of the representatives of the genus Chamaecytisus Link it is determined that 16 species of this genus naturally grow in the Ukraine flora. [Sect. Chrysochamaecytisus tzvel. C....

Gupta J.P.; Sharma B.B.L., 1988: District and block level planning for health. Need for district and block level health planning becomes much more explicit than before when the focus of developmental efforts is being geared towards decentralised planning. This paper, besides discussing the general framework, also draws upon...

Kawakatsu, M.; Kang, S.W., 1970: Annotated bibliography of Korean turbellarians. Korean J. Limnol. 2: 1969: 43-49

Melville R.V., 1967: Date of w fairleys practical observations on the south wales united kingdom coalfield. Journal of the Society for the Bibliography of Natural History 4(6): 298-299

Bagni, G., 1953: Recent methods of biometrical analysis of experimental results; application to a new drug tolerance test. Archivio di Farmacologia Sperimentale E Scienze Affini 81(3-4): 77-84

Shahina, F.; Maqbool, M.A., 1990: Distribution of corn cyst and cereal cyst nematodes in Pakistan. The distribution of Heterodera zeae and H. avenae in Pakistan is listed. There was a wide dispersal of these nematodes with H. zeae being more widely distributed than H. avenae especially in cereal crops.

Ramon Guerra, A.U., 1952: Clinical application of ACTH and cortisone in pediatrics. Archivos de Pediatria del Uruguay 23(7): 457-86; Contd

Allgaier, C.; Greber, R.; Hertting, G., 1991: Studies on the interaction between presynaptic alpha 2-adrenoceptors and adenosine A1 receptors located on noradrenergic nerve terminals. The aim of the present study was to obtain a more detailed understanding of the interaction between presynaptic alpha 2-adrenoceptors and A1 adenosine receptors mediating inhibition of noradrenaline release in the central nervous system. Slices of...

Berman, J.R.; Berman, L.A.; Werbin, T.J.; Goldstein, I., 2000: Female sexual dysfunction: anatomy, physiology, evaluation and treatment options. It has been estimated that up to 76% of women, depending upon their age, have complaints of sexual dysfunction, including decreased libido, vaginal dryness, pain with intercourse, decreased genital sensation and difficulty or inability to achieve...

Li,; Rhim, J.S.; Schlegel, R.; Vousden, K.H.; Colburn, N.H., 1998: Expression of dominant negative Jun inhibits elevated AP-1 and NF-kappaB transactivation and suppresses anchorage independent growth and HPV immortalized human keratinocytes. AP-1 transactivation appears to be required for mouse JB6 cell neoplastic transformation induced by the tumor promoter TPA or epidermal growth factor (EGF). Exposure to AP-1 transrepressing retinoids and glucocorticoids and expression of a dominan...