+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Solution structure of Alg13: the sugar donor subunit of a yeast N-acetylglucosamine transferase

, : Solution structure of Alg13: the sugar donor subunit of a yeast N-acetylglucosamine transferase. Structure 16(6): 965-975

The solution structure of Alg13, the glycosyl donor-binding domain of an important bipartite glycosyltransferase in the yeast Saccharomyces cerevisiae, is presented. This glycosyltransferase is unusual in that it is active only in the presence of a binding partner, Alg14. Alg13 is found to adopt a unique topology among glycosyltransferases. Rather than the conventional Rossmann fold found in all GT-B enzymes the N-terminal half of the protein is a Rossmann-like fold with a mixed parallel and antiparallel beta sheet. The Rossmann fold of the C-terminal half of Alg13 is conserved. However, although conventional GT-B enzymes usually possess three helices at the C terminus, only two helices are present in Alg13. Titration of Alg13 with both UDP-GIcNAc, the native glycosyl donor, and a paramagnetic mimic, UDP-TEMPO, shows that the interaction of Alg13 with the sugar donor is primarily through the residues in the C-terminal half of the protein.


PMID: 18547528

DOI: 10.1016/j.str.2008.03.010

Other references

Quadros L.P.; Melo J.H.G.; Grahn Y.; Steemans P.; L.H.risse A., 2000: Palynological characterization and dating of the Tiangua Formation, Serra Grande Group, Parnaiba Basin, northern Brazil. International Geological Congress, Abstracts = Congres Geologique International, Resumes 31(2000

Akşahin, Eğrul.; Yumrukçal, F.; Yüksel, H.Yalçın.; Doğruyol, Dğhan.; Celebi, L., 2010: Role of pathophysiology of patellofemoral instability in the treatment of spontaneous medial patellofemoral subluxation: a case report. Medial patellar subluxation is usually seen after lateral retinacular release. Spontaneous medial subluxation of the patella is a very rare condition. There are few reports in the literature on the pathophysiology of iatrogenic medial patellar sub...

Margitai Zsolt, 2002: Hydrogeological characterization of the Red River Formation, Williston Basin, Canada-USA. CSPG Annual Convention 2002(2002

Husak, R.; Blume-Peytaki, U.; Orfanos, C.E., 1999: Aleukemic leukemia cutis in an adolescent boy. New England Journal of Medicine 340(11): 893-894

Szobor, A.; Imre, G., 1965: Devincan-Therapy Of Cerebral Angiospasm. Therapia Hungarica 13: 9-16

Kuz'min, A.V.; Yatskov, S.V.; Orlov, A.N.; Ivanov, A.O., 1997: Domanik crisis in fauna development in the Frasnian sea basin at South Timan (North-Eastern Russian Platform). The faunistic complex of the Domanik Formation of Southern Timan is characterized by primary development of the planktonic forms and almost complete absence of benthos, that makes it significantly different from the faunistic assemblages from the...

Wiesmann, R., 1960: New methods of combatting flies in stables. As the house-fly becomes more and more resistant against practically all contact insecticides, other methods of control and other insecticides had to be found. Based on studies on the behavior and physiology of feeding of the fly poisons which hav...

Dyke, John Paul, 2000: Immune system catalyst. The present invention relates to a therapeutic composition for treating a wide variety of medical conditions, in particular a topical therapeutic composition for treatment of acne and other skin lesions/conditions. The invention also relates to me...

Ahall Karl Inge; Schoberg Hans, 1999: The 963 Ma Vinga Intrusion and post-compressional deformation in the Sveconorwegian Orogen, SW Sweden. GFF 121(2): 101-106

Littleton, D.; Marsalis, S.; Bliss, D.Zimmaro., 2004: Searching the literature by design. Current nursing knowledge has been generated by studies whose designs range from exploratory to experimental. Searching the literature for a study illustrating a particular design can be a perplexing process. This article explains strategies that...