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The transformation of insect antifreezing protein for Sauropus androgynus mediated by Agrobacterium


, : The transformation of insect antifreezing protein for Sauropus androgynus mediated by Agrobacterium. Bulletin Of Botanical Research: 5, 564-567, 573

In order to improve the cold tolerance in this vegetable, a genetic transformation system for <i>S. androgynus</i> was established. The cDNA <i>Mpafp</i>149 of <i>Microdera punctipennis dzungarica</i> was used as target gene to obtain cold tolerant transgenic <i>S. androgynus</i> plants. The stems of <i>S. androgynus</i> were transformed with <i>Agrobacterium tumefaciens</i> EHA105 strain which harbors plasmid pCAMBIA-1302-<i>Mpafp</i>149.

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