geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Activated notch alters development of the dopaminergic neuron pattern in the ventral spinal cord


, : Activated notch alters development of the dopaminergic neuron pattern in the ventral spinal cord. Society for Neuroscience Abstracts 26(1-2): Abstract No -22 13

The Notch signaling pathway is an important regulator of cell fate in the neural plate. We have previously shown that a population of dopaminergic neurons differentiates in a Notch-like lateral inhibitory pattern in the spinal cord of the frog Xenopus laevis during embryonic, larval and metamorphic development. To test whether Notch signaling was involved in generating this pattern, we overexpressed a constitutively active form of Xenopus Notch (XotchDELTAE) in developing frog embryos. Since dopaminergic cells form two longitudinal columns on either side of the ventral spinal cord, we altered only one by injecting RNAs for XotchDELTAE and a marker gene (GFP) into one blastomere at the two-cell stage. Animals showed differences on the injected (green) side including reduction of the eye and enlargement of the brain that were not apparent in animals injected with GFP alone. The lateral inhibitory pattern of dopaminergic neurons appeared normal on both control and experimental sides of the spinal cord. However, analysis of the spacing between dopaminergic neurons showed that there were significantly fewer cells in experimental columns. This is consistent with a role for Notch signaling in specifying fate within a specific population of neurons. Analysis at a later stage of development showed an increase in cell density, but the difference between experimental and control columns remained evident. Thus, alteration of Notch signaling during early development apparently had a permanent effect on the dopaminergic neuron population.

US$29.90


Other references

Taylor, A.F., 2005: Functional osteoblastic ionotropic glutamate receptors are a prerequisite for bone formation. Many studies have now demonstrated that osteoblasts express the protein components necessary for functional neuronal-like glutamatergic signalling to occur, and as a result a physiological role for receptor mediated osteoblastic glutamate signalli...

Tench, L.H.rper, S., 1987: Oxygen-bleaching practices and benefits: an overview. Tappi journal 70(11): 55-61

Marczynski, B.; Merget, R.; Chilian, B.; Korn, M.; Rabstein, S.; Bruening, T., 2003: Changes in low molecular DNA fragmentation of white blood cells after chamber exposure of workers to aromatic and aliphatic diisocyanates. Toxicology Letters (Shannon) 144(Suppl 1): s159, September

Margulis, H., 1946: Remarks on the chemical activity of soil constituents.. Experimental evidence is adduced to disprove the idea that the chemical activity of the soil is entirely concentrated in the clay part of the soil. The chemical activity of the soil was measured by determining the buffer capacity of the different...

Broyer, M.; Jouvet, P.; Niaudet, P.; Daudon, M.; Revillon, Y., 1996: Management of oxalosis. Oxalosis is the final stage of primary hyperoxaluria type I (PHI) when reduction of GFR produces oxalate accumulation. It involves bones, arteries, eyes, heart, nerves, etc. The management of oxalosis starts with prevention of nephrocalcinosis and...

Coppola, A.R., 1979: Anterior cervical discectomy without fusion. Virginia Medical 106(5): 297-298

Yoshino A.; Yoshida T.; Takahashi K.; Ueda I., 1989: Anesthetic effect on interfacial water a deuterium nmr study of water in oil reversed micelles. Anesthetic effects upon surface-bound water were investigated with 2H2O NMR spectroscopy in water-in-oil reversed micelles consisting of 2H2O, n-decane, and glycerol-.alpha.-monooleate (GMO). The spin-lattice relaxation rate, R1 (= 1/T1), of the 2...

Hotz-Behofsits, C.M.; Walley, M.J.M.; Simpson, R.; Bjarnason, I.T., 2004: COX-1, COX-2 and the topical effect in NSAID-induced enteropathy. The side effects of NSAIDs are equally evident in the stomach and the small bowel. The latter is increasingly seen as being clinically significant, contributing substantially to the iron-deficiency anaemia that is so common in patients with rheuma...

Uchida, Y.; Takeuchi, K.; Kanai, M.; Sakurai, T.; Kameda, N.; Hiruta, K.; Tachihara, K.; Nagaki, K., 1999: Glistening yellow coronary plaque, a vulnerable plaque, and its pathological correlation. Circulation 100(18 SUPPL ): I 518, Nov 2

Eros, E.; Hajós, A., 2011: Prevention of perinatal depression and anxiety with periconceptional care. In the public mind, pregnancy, childbirth and newborn care is one of the most wonderful living experiences. However, epidemiological studies have shown that the risks of psychiatric disorders in the perinatal period are multiplying. Pregnancy enta...