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Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays

, : Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays. Official Gazette of the United States Patent & Trademark Office Patents 1252(2), Nov 13

Methods and apparatus for evanescent light fluoroimmunoassays are disclosed. The apparatus employs a planar waveguide with an integral semi-cylindrical lens, and has multi-analyte features and calibration features, along with improved evanescent field intensity. A preferred embodiment of the biosensor and assay method have patches of capture molecules each specific for a different analyte disposed adjacent within a single reservoir. The capture molecules are immobilized to the patches on the waveguide surface by site-specific coupling of thiol groups on the capture molecules to photo-affinity crosslinkers which in turn are coupled to the waveguide surface or to a non-specific-binding-resistant coating on the surface. The patches of different antibodies are produced by selectively irradiating a portion of the waveguide surface during the process of coupling the photo-affinity crosslinkers the selective irradiation involving a mask, a laser light source, or the like.


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Karki, Madhav, 2003: Organic conversion & certification: A strategy for improved value-addition and marketing of medicinal plants products in the Himalayas. The Himalayan mountains are extremely rich in medicinal and aromatic plants (MAPs) wealth. However, due to years of unwise use, the availability of medicinal plant materials in desired quality, quantity, time, and place have become difficult raisi...

Edozien, J.C., 1958: Biochemical normals in Nigerians. 1. Blood. Blood samples were obtained from Nigerians, 301 men and 51 women aged from 20 to 50 years. Estimations were made of 28 commonly investigated constituents or functions of the blood and mean values are tabulated. Except for serum proteins, cephalin...

Wichowski, W.A.; Holmes, G.W., 1960: Traumatic diaphragmatic hernia: diagnosis and treatment. Industrial Medicine & Surgery 29: 577-579

Ryan, P.B.; Lee-Wilk, T.; Kok, B.C.; Wilk, J.E., 2011: Interdisciplinary rehabilitation of mild TBI and PTSD: a case report. Prevalence of mild traumatic brain injury (mTBI) or concussion on the battlefield in Iraq/Afghanistan has resulted in its designation as a 'signature injury'. Civilian studies have shown that negative expectations for recovery may lead t...

Gregori, P., 1964: Comparative Studies Of The Asthmatic Disposition In Pollen Allergy By Means Of The Acetylcholine Test And Allergen Loading Tests. Zeitschrift für Arztliche Fortbildung 58: 1297-1301

Armstrong, E., 2002: Compounding for geriatric patients. International Journal of Pharmaceutical Compounding 6(3): 168-170

Hildebrand Ernst E., 1980: Possibilities and limits of forest and agricultural use of tropical regions using the example of the Amazonas region. Jahreshefte der Gesellschaft fuer Naturkunde in Wuerttemberg 135: 18-31

Bidinger, P.; Nag, B., 1981: The role of pigeonpeas in village diets in the semi-arid tropics of South India. Economics Program Progress Report International Crops Research Institute for the Semi Arid Tropics: 981 23

Spalding, V.M., 1900: Progress In Forestry Under State Control. Science 12(313): 977-989

Wollmuth, L.P., 1994: Mechanism of Ba2+ block of M-like K channels of rod photoreceptors of tiger salamanders. I-Kx is a voltage-dependent K+ current in the inner segment of rod photoreceptors that shows many similarities to M-current. The depression of I-Kx by external Ba-2+ was studied with whole-cell voltage clamp. Ba-2+ reduced the conductance and volt...