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Calcium signalling and synaptic plasticity in cerebellar Purkinje cells of mormyrid FISH

, : Calcium signalling and synaptic plasticity in cerebellar Purkinje cells of mormyrid FISH. Society for Neuroscience Abstract Viewer & Itinerary Planner : Abstract No 73 7

In mammalian Purkinje cells (PCs), simultaneous parallel fiber (PF) and climbing fiber (CF) activation is required for the induction of PF-LTD. The contribution of the CF consists of complex spike-associated, dendritic Ca signals. Interestingly, the architecture of the dendritic tree is quite different in mormyrid PCs from that in mammalian PCs. The smooth proximal dendrites, which are the input sites of CF synapses, do not enter into the molecular layer where PFs terminate, leading to a pronounced separation between PF and CF synapses. To assess the interaction of PF-and CF-evoked Ca signals under these conditions, we performed patch-clamp recordings from PCs in cerebellar slices (200um) obtained from the elephant nose fish Gnathonemus petersii. In this preparation, PF-LTD can be obtained by a transient increase of the PF-pulse duration (0.1 to 0.3ms; LTD in 15/18 cells). A subsequent increase in the pulse frequency (to 1-4Hz) potentiates the depressed PF-responses (8/14 cells). To monitor dendritic Ca signals, we used a cooled CCD camera and the Ca indicator Oregon Green BAPTA-2 (200uM). CF stimulation evoked Ca signals in both, the smooth proximal dendrites as well as at the PF input sites on the secondary dendrites (n=13). PF stimulation caused dendritic Ca transients as well (n=7). When CF and PF inputs were coactivated, the Ca signals measured within the PF territory reached larger amplitudes than those measured with either stimulation alone (n=7). Ca spikes evoked by depolarizing current pulses resulted in large dendritic Ca transients as well (n=2). Whereas PF-LTD/LTP can be obtained with PF stimulation alone, our imaging data show that paired CF activity enhances dendritic Ca signals and suggest that CF activity (and Ca spikes) might influence PF synaptic plasticity.


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