+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Comparison of real-time PCR and culture of Porphyromonas gingivalis from subgingival plaque

, : Comparison of real-time PCR and culture of Porphyromonas gingivalis from subgingival plaque. Abstracts of the General Meeting of the American Society for Microbiology 103: C-189

Background: Quantitative anaerobic culturing is used to detect Porphyromonas gingivalis in periodontal pockets but this method lacks sufficient sensitivity and sometimes specificity. It is also laborious and some periodontal microorganisms are either uncultivable or require very specific growth conditions. We designed a Real-Time PCR assay for sensitive, specific, efficient, reproducible and rapid detection and quantification of P. gingivalis in subgingival plaque samples. The quantitative anaerobic culture technique was used as a reference to test for the efficacy of the Real-Time PCR. Methods: a Real-Time PCR assay was developed on the 16s rRNA sequences of P. gingivalis. The PCR was validated on pure cultures of P. gingivalis and other oral and non-oral bacteria. Subsequently subgingival plaque samples from 259 adult patients with periodontitis were tested with anaerobic culture and Real-Time PCR simultaneously. Results: The primers for P. gingivalis demonstrated specific amplification of only P. gingivalis DNA. The PCR detection sensitivity was 1 CFU. P. gingivalis was detected in 111 (43%) subgingival plaque samples by the culture method and in 118 samples (46%) by Real-Time PCR. The culture assay failed to detect P. gingivalis in seven samples from which the organism was detected by Real-Time PCR. Another twenty samples were also found to be positive (<10000 CFU/ml subgingival plaque sample) with the PCR and negative with the culture. This however was due to the detection limit of the culture (104 CFU/ml subgingival plaque sample). Conclusion: Real-Time PCR confirms quantitative culture of P. gingivalis and offers significant advantages with respect to rapidity and sensitivity to detect P. gingivalis in subgingival plaque.


Other references

Voss, H., 1993: Farm labor and immigration policy. Outlook 221-227

Zambernard J., 1982: Intra cellular copper levels in the hamster uterine luminal epithelium exposed to the copper intra uterine contraceptive device. Anatomical Record: 163

Zobel, M.; Schilling, J., 1964: On The Formation Of Water-Soluble Substances Following Storage Of Washed Perfol Bottles. Die Pharmazie 19: 273-276

Szadurska, M., 1967: Attempt of evaluation of occupational exposure in factories of Wrocław. Zdrowie Publiczne 2: 173-177

Hall B.L.; Denton G.H.; Overturf B., 2001: Glacial Lake Wright, a high-level Antarctic lake during the LGM and early Holocene. We report evidence of a large proglacial lake (Glacial Lake Wright) that existed in Wright Valley in the McMurdo Dry Valleys region of Antarctica at the last glacial maximum (LGM) and in the early Holocene. At its highstands, Glacial Lake Wright w...

Miyamae, M.; Suzuki, I., 1983: Approach to some famous painters' pathography by computer picture analysis. Iyo Denshi to Seitai Kogaku. Japanese Journal of Medical Electronics and Biological Engineering 21(4): 264-265

Turbiak, J.; Miatkowski, Z.; Sotysik, A., 2004: Assessment of mineralisation rate of nitrogen organic compounds by methods of aerobic and anaerobic incubation in mineral-muck soils. The potential mineralization rates of nitrogen organic compounds in mineral-muck soils after soil incubation under aerobic and anaerobic conditions were compared, and the relationships between the mineralization rate of nitrogen organic compounds...

Barreau, J.M.; Bukstein, D.A.; Luskin, A.T., 2000: Implementation strategy to improve cost-effective therapy of allergic rhinitis. Journal of Allergy & Clinical Immunology 105(1 part 2): S4, Jan

Sugawara, E.; Togashi, Y.; Kuroda, N.; Sakata, S.; Hatano, S.; Asaka, R.; Yuasa, T.; Yonese, J.; Kitagawa, M.; Mano, H.; Ishikawa, Y.; Takeuchi, K., 2012: Identification of anaplastic lymphoma kinase fusions in renal cancer: large-scale immunohistochemical screening by the intercalated antibody-enhanced polymer method. Several promising molecular-targeted drugs are used for advanced renal cancers. However, complete remission is rarely achieved, because none of the drugs targets a key molecule that is specific to the cancer, or is associated with "oncogene a...

Satpaeva R.A., 1966: The state of the oral cavity and organs of mastication in patients with neopl esophageal cancer. Trudy Alma-Atinskogo Meditsinskogo Instituta 23: 368-372