+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Dietary fish oil and pectin protect against oxidative DNA damage in rat intestinal epithelial cells due to heightened apoptosis induced by reactive oxygen species

, : Dietary fish oil and pectin protect against oxidative DNA damage in rat intestinal epithelial cells due to heightened apoptosis induced by reactive oxygen species. FASEB Journal 16(4): A371, March 20

Reactive oxygen species (ROS) can lead to tumorigenesis by damaging DNA, or alternatively protect against tumor development by upregulating apoptosis to remove DNA damaged cells. Since we have shown a fish oil/pectin diet protects against experimentally-induced colon cancer and elevates ROS, compared to a corn oil/cellulose diet, we hypothesized that elevated ROS upregulates apoptosis and consequently decreases oxidative DNA damage in intestinal epithelial cells. To test this, 60 male SD rats were provided one of four diets (15% corn or fish oil, and 6% pectin or cellulose) for 2 wk prior to measurement of ROS by fluorescence microscopy, apoptosis by TUNEL assay, and oxidative DNA damage by a FLARE assay (enzyme-based single cell gel electrophoresis). A fish oil/pectin diet yielded 3-fold higher levels of apoptosis (p<0.0001) than a corn oil/cellulose diet. Overall, there was an exponential increase in apoptosis as ROS increased (p<0.005), yet only in a fish oil/pectin diet did elevated ROS correspond to decreased levels of oxidative DNA damage (p<0.0001). While many view elevated ROS as undesirable due to their damaging effects on DNA, these data suggest increased ROS may actually protect against oxidative DNA damage by providing a mechanism for fish oil/pectin to induce apoptosis thereby removing damaged cells. These findings may also partially explain this diet's protective effect in colon carcinogenesis.


Other references

Maehara, S.; Nakajima, K.; Utsumi, A.; Makino, Y.; Uzawa, Y., 1974: Status of nursing school graduates. 1. Japanese Journal of Nursing 26(6): 49-58

Dittrich, W., 1978: Japanese begonia, a little known shade shrub. Gartenpraxis 3 124-125

Rocha, G.; Fraga, S., 1962: Sea urchin granuloma of the skin. Archives of Dermatology 85: 406-408

Anonymous, 2000: Annual report 1998/99 - Federal Research Institute of Agricultural Ecology and Agriculture. This is the annual report of the research activities of the Federal Research Institute of Agricultural Ecology and Agriculture in 1998/99. It is divided into the following research areas: (1) Landscape ecology on biodiversity; (2) Field and forage...

Schuler, B.; Müller-Späth, S.; Soranno, A.; Nettels, D., 2012: Application of confocal single-molecule FRET to intrinsically disordered proteins. Intrinsically disordered proteins (IDPs) are characterized by a large degree of conformational heterogeneity. In such cases, classical experimental methods often yield only mean values, averaged over the entire ensemble of molecules. The microscop...

Hu, Q.; Yang, C.; Wang, Q.; Zeng, H.; Qin, W., 2016: Demethylzeylasteral (T-96) Treatment Ameliorates Mice Lupus Nephritis Accompanied by Inhibiting Activation of NF-κB Pathway. Inflammation plays a vital role in the pathogenesis in lupus nephritis (LN), which is largely attributable to the activation of nuclear factor kappa B (NF-κB) signal pathway. NF-κB up-regulates pro-inflammatory mediators, such as TNF-α, cyclo-o...

Ocker, H.; Wenzel, V.; Schmucker, P.; Steinfath, M.; Dörges, V., 2002: A comparison of the laryngeal tube with the laryngeal mask airway during routine surgical procedures. The laryngeal mask airway (LMA; Laryngeal Mask Company, Henley-on-Thames, UK) is an established airway device, whereas the laryngeal tube (LT) is relatively new and therefore not as well investigated. Therefore, the purpose of the present prospect...

Rodrigues, A.S.; Gomez, B.J., 1999: Copulatory process in Oxychilus (Drouetia) atlanticus (Morelet and Drouet, 1857) (Pulmonata: Zonitidae). Oxychilus (Drouetia) atlanticus (Pulmonata: Zonitidae) is an endemic hermaphroditic species from Sao Miguel island (Azores). The copulatory process is a mutually simultaneous event and takes about 24 h from the beginning of penetration until the d...

Kohn, A.; Oppenheimer, J., R., 1991: Fortune or failure (book review). The Quarterly Review of Biology 66: 8

Gaudet, R.; Bohm, A.; Sigler, P., B., 1996: Crystal structure at 2.4 A resolution of the complex of transducin beta-gamma and its regulator, phosducin. The crystal structure of transducin's beta-gamma subunits complexed with phosducin, which regulates G-t-beta-gamma activity, has been solved to 2.4 ANG resolution. Phosducin has two domains that wrap around G-t-beta-gamma to form an extensive...