geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effects of higher-order nuclear structure and Rad51 overexpression on radiation-induced chromosome rearrangements


, : Effects of higher-order nuclear structure and Rad51 overexpression on radiation-induced chromosome rearrangements. Cytogenetic and Genome Research 98(4): 265-269

The microchromosomes (MICs) in chicken DT40 lymphocytes are usually clustered in the center of the nucleus, whereas the macrochromosomes (MACs) are preferentially located toward the nuclear periphery. This compartmentalized architecture of the nucleus is associated with a low frequency of translocations between MICs and MACs after induction of DNA breaks by a radiation track(s). In contrast, the MICs in chick embryo fibroblasts (CEFs) tend to be located throughout the entire nuclear volume. The resulting side-to-side arrangement of MIC and MAC territories favors radiation-induced MIC/MAC translocations, which occur more frequently in CEF cells than MIC/MIC or MAC/MAC rearrangements. Collectively, our results suggest that preformed physical contacts are a prerequisite for the generation of chromosome rearrangements through recombinational repair of DNA damage. Cell type-specific higher-order nuclear organization may prevent or stimulate the formation of particular chromosome aberrations in pathology and evolution. Ectopic expression of the recombination protein Rad51 can protect cells from radiation-induced translocations. The repair activity of overexpressed Rad51 is more important for cells that are irradiated in S/G(2) phase than for cells in G(1) phase. Evidently, homologous recombination between sister chromatids of a replicated chromosome is more frequent than that between homologous or heterologous chromosomes during G(1) phase.

US$19.90

PMID: 12826751

DOI: 71046


Other references

Jagt, JWM.; Michels, GPH., 1987: Cyclaster platornatus Kutscher, 1978: an addition to the echinoid fauna from the Late Maastrichtian of NE Belgium. Two fairly well preserved specimens of the brissid echinoid Cyclaster platornatus Kutscher, 1978 are described from the late Maastrichtian Vijlen Member (Gulpen Formation) as exposed in the SA Ciments Portland Liegeois (CPL) quarry at Haccourt, mu...

Janse, J.H.; Ligtvoet, W.; Tol, S. van; Bresser, A.H.M., 2001: A model study on the role of wetland zones in lake eutrophication and restoration. Shallow lakes respond in different ways to changes in nutrient loading (nitrogen, phosphorus). These lakes may be in two different states: turbid, dominated by phytoplankton, and clear, dominated by submerged macrophytes. Both states are self-stab...

Spittler, J.; Keeling, J., 2016: Water Polo Injuries and Training Methods. NlmCategory="UNASSIGNED">Water polo is a unique team sport combining swimming sprints and eggbeater kicking, frequent overhead movements and throwing, and regular physical contact with minimal protective equipment. Accordingly, a wide...

Cheng, W-Chun.; Chang, J-Chian.; Chang, C-Ping.; Su, Y.; Tu, T-Ming., 2008: A Fixed-Threshold Approach to Generate High-Resolution Vegetation Maps for IKONOS Imagery. NlmCategory="UNASSIGNED">Vegetation distribution maps from remote sensors play an important role in urban planning, environmental protecting and related policy making. The normalized difference vegetation index (NDVI) is the most popu...

Satpute, S.R.; Rajaiah, R.; Polumuri, S.K.; Moudgil, K.D., 2009: Tolerization with Hsp65 induces protection against adjuvant-induced arthritis by modulating the antigen-directed interferon-gamma, interleukin-17, and antibody responses. Arthritis and Rheumatism 60(1): 103-113

Napolitano, C.; Schwartz, P.J.; Bianchi, L.; Dennis, A.; D.F.sco, M.; Brown, A.M.; Casari, G.; Priori, S.G., 1998: A recessive variant of Romano ward long QT syndrome. European Heart Journal 19(ABST SUPPL ): 424, Aug

Vickery H.B., 1972: A chemist among plants. Machlis, Leonard, Winslow Rs And Roderic B. Park (ed.). Annual Review Of Plant Physiology, Vol. 23. Xi 505p. Illus. Annual Reviews Inc: Palo Alto, Calif., U.s.a. 1-28

Elmqvist, B.; Khatir, A.R., 2007: The possibilities of bush fallows with changing roles of agriculture - An analysis combining remote sensing and interview data from Sudanese drylands. The lengths of fallows have decreased in many parts of the Sahel due to agricultural expansion, which can have negative impacts on crop production when few other ways to improve soil fertility exist. However, the dynamics of agricultural expansio...

Lim, K.H.H.; Caputo, M.; Ascione, R.; Wild, J.; West, R.; Angelini, G.D.; Bryan, A.J., 2002: Prospective randomized comparison of CarboMedics and St Jude Medical bileaflet mechanical heart valve prostheses: an interim report. This is a midterm report of a study comparing the clinical performance of CarboMedics and St Jude Medical heart valve prostheses through a projected 10-year period. Between 1992 and 1996, a total of 485 patients undergoing mechanical valve replace...

Samy, R.N., 2016: Caring for the whole patient in otology/neurotology. Current Opinion in Otolaryngology & Head and Neck Surgery 23(5): 329-329