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Expression of porcine myocilin and optineurin in trabecular meshwork cells and astrocytes from optic nerve head


, : Expression of porcine myocilin and optineurin in trabecular meshwork cells and astrocytes from optic nerve head. ARVO Annual Meeting Abstract Search & Program Planner : Abstract No 1115

Purpose: Recently, optineurin (OPTN), a new gene responsible for primary open-angle glaucoma and normal tension glaucoma was identified (Sarfarazi et al. Science 2002). To compare the expression of OPTN with myocilin (MYOC) we determined the complete sequence of porcine OPTN cDNA and performed quantitative-PCR analysis on two porcine primary cells, trabecular meshwork cells (TMC) and astrocytes, cultured under various stimuli and stresses, which mimic the glaucoma cellular conditions. Methods: Coding region of porcine OPTN cDNA was amplified by RT-PCR from porcine TMC total RNA using primers designed from conserved sequence between human, mouse, and rat. The lacking 5' and 3' cDNA ends were amplified by 5' and 3' RACE method respectively. Total RNA was isolated from cultured cells treated with dexamethasone (500nM, for 2weeks), hydrostatic pressure (+30mmHg, 72h) or hypoxia (7% O2, 72h). The expression level of the OPTN and MYOC in TMC and astrocytes were analyzed by real-time quantitative PCR (GeneAmp5700, PE Biosystems, Inc.). Total RNA used for quantitation was treated with DNase prier to measurement. Results: Porcine OPTN protein was composed of 574 amino acids, which was 84% identical with that of human OPTN protein. Treatment with dexamethasone increased MYOC expression by 8.2-fold and 5.5-fold in TMC and astrocytes respectively, while OPTN was suppressed to 48% in astrocytes and 68% in TMC. Incubation under hypoxia showed significant decrease for MYOC in both cells, while OPTN transcript was not affected. Hydrostatic pressure did not affect both genes in both cells. Conclusions: Nucleotide sequence of full-length porcine OPTN cDNA was determined and showed high degree of homology with human. OPTN expression was compared with MYOC under stimuli or stress in two porcine primary cells. Expression of OPTN was suppressed by dexamethasone but no effect was observed under hypoxia or hydrostatic pressure.

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Other references

Davis, W.T., 1939: New Cicadas from North America and the West Indies. Journal of the New York Entomological Society, 47: 287-302

Surovtsev, V.; Tret' yakov, N.; Kuznetsov, A., 1972: Light regime - a reserve for increasing production in fowl. 57 000 female Leghorn chicks (9500/group) were reared in cage batteries on a light regime in which the daily photoperiod and light intensity were as follows: (1) 24 h/10 lux, (2) 24 h/60 lux, (3) 18 h/10 lux, (4) 18 h/60 lux, (5) 10 h/10 lux, (6)...

Long Ashton Leaf Group (Engl), 1970: Physical characteristics of leaf surfaces as revealed by the stereo scan electron microscope. Proceedings of the International Horticultural Congress 1: 73

Gerard V.B., 1969: A note on the Yamazaki resistivity variometer. Electrical resistivity of rock, stress function, earthquake association.

Singh, R.P.; Bandhuvula Padmavathi; Rao, A.R., 2000: Modulatory influence of Adhatoda vesica (Justicia adhatoda) leaf extract on the enzymes of xenobiotic metabolism, antioxidant status and lipid peroxidation in mice. The effect of two different doses (50 and 100 mg/kg body wt/day for 14 days) of 80% ethanolic extract of the leaves of Adhatoda vesica were examined on drug metabolizing phase I and phase II enzymes, antioxidant enzymes, glutathione content, lacta...

Anonymous, 2001: HIV perception as legal reality: what the courts say about the ADA. An employer can be held liable for HIV discrimination under the Americans with Disabilities Act (ADA) even if the employee is not infected with the virus. Being "regarded as" having an impairment, such as HIV infection, qualifies a perso...

Whitten, B.K.; Janoski, A.H., 1969: Effects of high altitude and diet on lipid components of human serum. Federation Proceedings 28(3): 983-986

Piau J.P.; Fischer S.; Delaunay J.; Beaumont C.; Schapira G., 1981: Hormonally stimulated adenylate cyclase and protein kinase in membranes of rabbit erythroid cells separated according to density. Dumont, J E , P Greengard And G A Robison (Ed ) Advances in Cyclic Nucleotide Research, Vol 14 4th International Conference, Brussels, Belgium, July 22-26, 1980 Xxxi+724p Raven Press: New York, N Y , Usa Illus P674

Fan, C.Y.; Sakuraba, H.; Yeldandi, A.V.; Rao, M.S.; Reddy, J.K., 1997: Development of hepatocellular carcinoma in mice with disrupted peroxisomal fatty acyl-CoA oxidase gene. FASEB Journal 11(3): A227

Dressler D.P.; Skornik W.A., 1971: Patho physiology of thermal respiratory injury. Matter, P , T L Barclay And Z Konickova Research in Burns Symposium 730p Illus Hans Huber Publishers: Stuttgart, West Germany 457-460