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Hippocampal mRNA profiles of transgenic mice expressing comparable levels of wildtype or Alzheimer-mutant human amyloid protein precursors

, : Hippocampal mRNA profiles of transgenic mice expressing comparable levels of wildtype or Alzheimer-mutant human amyloid protein precursors. Society for Neuroscience Abstracts 27(1): 332

Increased cerebral levels of beta-amyloid peptides (Abeta) are associated with Alzheimer's disease (AD). Mutations in the human beta-amyloid protein precursor (hAPP) alter Abeta production and cause autosomal dominant familial AD (FAD). How Abeta induces neurodegeneration or cognitive decline is unknown. Previously, we showed that age-dependent hippocampal decreases in presynaptic terminal density and synaptic transmission strength in hAPP transgenic (tg) mice depended on Abeta levels but not on Abeta deposition into plaques (J. Neurosci. 20: 4050). hAPP-FAD tg mice typically had higher Abeta levels and more prominent synaptic deficits than hAPP-wildtype (WT) tg mice. We hypothesized that the underlying mechanisms would be reflected, in part, in changes of hippocampal gene expression. Hippocampal RNA from individual hAPP-WT and hAPP-FAD mice was analyzed with microarrays representing over 13,000 sequences. Potentially significant differences between groups were found for 367 sequences. The 20 genes and 14 ESTs with the greatest changes belonged to the following clusters: extracellular matrix components, adhesion molecules, enzymes involved in neurotransmitter turnover, transcription factors, growth factors, and synaptic proteins. We are confirming these molecular leads by quantitative fluorogenic PCR and immunochemistry. In parallel studies, we are examining the precise role of validated targets in Abeta-dependent neurodegeneration and cognitive decline.


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Treml, F.; Pikula, J.; Bandouchova, H.; Horakova, J., 2007: European brown hare as a potential source of zoonotic agents. Recently, there has been a growing interest in checking the state of health of European brown hares in hunting grounds, because they are susceptible bioindicators of environmental change and have shown a dramatic decline in populations across Euro...

Palmer, E.D., 1962: Further experiences with Saint's triad (hiatus hernia, gallstones and diverticulosis coli). American Journal of the Medical Sciences 244: 70-74

Migotin, S.V.; Stepanenko, V.V.; Lipkan, G.N.; Korotkoruchko, A.G.; Vishnevskaia, M.G., 1992: Evaluation of the uptake of crystalline amino acid preparations based on the concentration of urea in the blood and tissues. The assimilation of polyamine, levamine and "new" alvesin has been experimentally assessed by blood and tissue urea concentrations. It has been established that polyamine was best assimilated and was followed by levamine and "new&qu...

Kleine, R., 1939: Entomological results from the Swedish expedition1934to Burma and British India. Coleoptera: Brenthidae and Lycidae, gesammelt von Herrn Rene MalaiseZool., Stockholm. ; 31A: 23 pp.

Bonilla Marti, E., 1979: The mamma in gynecology. Revista Espanola de Obstetricia Y Ginecologia 38(247): 355-369

Fajersson, F., 1968: WeibulPs Pompe spring wheat, a new high-quality variety. Pompe, a medium-early variety, was selected from a cross of Svenno x Ring. Varietal trials showed that Pompe outyielded Svenno, Ring and Prins in all regions of Sweden. The bulk weight is the same as in Svenno and greater than in Ring. The 1000-ke...

Rothrock, J.F., 2011: Occipital nerve blocks. Occipital nerve block (ONB) is a promising treatment for headaches. Its indications, selection criteria, and best techniques are not clear, however. To summarize in narrative format what is known about ONBs and what needs to be learned. MD Consult...

Mayandon, T.H.; Gallo, P., 1961: Eimerosis de las palomas (Columba livia domestica) en Venezuela por Eimeria labbeana Pinto, 1928. Revista de Medicina Veterinaria y Parasitologia Maracay, 18: 17-21

Schmid, E.E.; Velaudapillai, T., 1954: A Providence organism related to S. milwaukee. The authors describe an organism belonging to the Providence group, possessing the somatic (XLIII) antigen of Salmonella milwaukee.

Jäckel, W.H.; Mau, W.; Zink, A.; Beyer, W.; Droste, U.; Engel, M.; Genth, E., 2005: Routine report on the medical rehabilitation of patients with musculoskeletal diseases. The commission "Rehabilitation" of the German Society of Rheumatology compiled a data set for a routine report of the rehabilitation system for muskuloskeletal diseases. More than 250 rehabilitation hospitals offer inpatient rehabilitati...