+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification of a minimal promoter sequence for the human N-acetyltransferase Type I gene that binds AP-1 and YY-1

, : Identification of a minimal promoter sequence for the human N-acetyltransferase Type I gene that binds AP-1 and YY-1. Biochemical Journal 376(2): 441-448, 1 December

Human N-acetyltransferase Type I (NAT1) catalyses the acetylation of many aromatic amine and hydrazine compounds and it has been implicated in the catabolism of folic acid. The enzyme is widely expressed in the body, although there are considerable differences in the level of activity between tissues. A search of the mRNA databases revealed the presence of several NAT1 transcripts in human tissue that appear to be derived from different promoters. Because little is known about NAT1 gene regulation, the present study was undertaken to characterize one of the putative promoter sequences of the NAT1 gene located just upstream of the coding region. We show with reverse-transcriptase PCR that mRNA transcribed from this promoter (Promoter I) is present in a variety of human cell-lines, but not in quiescent peripheral blood mononuclear cells. Using deletion mutant constructs, we identified a 20 bp sequence located 245 bases upstream of the translation start site which was sufficient for basal NAT1 expression. It comprised an AP-1 (activator protein 1)-binding site, flanked on either side by a TCATT motif. Mutational analysis showed that the AP-1 site and the 3' TCATT sequence were necessary for gene expression, whereas the 5' TCATT appeared to attenuate promoter activity. Electromobility shift assays revealed two specific bands made up by complexes of c-Fos/Fra, c-Jun, YY-1 (Yin and Yang 1) and possibly Oct-1. PMA treatment enhanced expression from the NAT1 promoter via the AP-1-binding site. Furthermore, in peripheral blood mononuclear cells, PMA increased endogenous NAT1 activity and induced mRNA expression from Promoter I, suggesting that it is functional in vivo.


Other references

Moralez, A.M.; Maile, L.A.; Clarke, J.; Busby, W.H.; Clemmons, D.R., 2005: Insulin-like growth factor binding protein-5 (IGFBP-5) interacts with thrombospondin-1 to induce negative regulatory effects on IGF-I actions. Insulin-like growth factor binding protein-5 (IGFBP-5) and thrombospondin-1 (TS-1) are both present in extracellular matrix (ECM). Both proteins have been shown to bind to one another with high affinity. The purpose of these studies was to determi...

Welander, L., 1978: Diagnosis of nerve--muscle diseases (1): muscular diseases in adults. Lakartidningen 75(9): 805-829

Ismail, F.; Davies, M., 2006: Integrated monitoring and analysis for early warning of patient deterioration. Recently there has been an upsurge of interest in strategies for detecting at-risk patients in order to trigger the timely intervention of a Medical Emergency Team (MET), also known as a Rapid Response Team (RRT). We review a real-time automated s...

Bosshard, H.H., 1961: Creosote impregnation of Beech and Oak sleepers at 100 and 130 degrees C. I: Microscopical investigations on changes in structure and moisture content.. Beech and Oak sleepers, freshly cut or dry, were impregnated with creosote by the Ruping process at 100 and 130 degrees C. The m.c. and structure of the sleepers were then studied with photomicrographs. Even at 100 degrees , both species showed st...

Capozzi, F.; Bordoni, A., 2012: Foodomics: a new comprehensive approach to food and nutrition. In the past 20 years, the scientific community has faced a great development in different fields due to the development of high-throughput, omics technologies. Starting from the four major types of omics measurements (genomics, transcriptomics, p...

Lei, M.; Honjo, H.; Kodama, I.; Boyett, M.R., 2001: Heterogeneous expression of the delayed-rectifier K+ currents iK,r and iK,s in rabbit sinoatrial node cells. 1. The electrical activity of sinoatrial node cells is heterogeneous. To understand the reasons for this, the density of the delayed-rectifier K+ current and its two components, i(K,r) and i(K,s), as a function of the size (as measured by cell cap...

Jones Lecointe A.; Rose S.P.R.; Sinha A.K., 1974: Sub cellular localization of proteins labeled in visual cortex on 1st exposure of dark reared rats to light. Biochemical Society Transactions 2(2): 238-239

Tverskaya M.Ya, 1975: Hygienic regulation of the use of some stabilizers in food brands of poly vinyl chloride compounds. Gigiena I Sanitariya: 28

Oles, A.; Kurzeja, K.; Sulinski, S., 1956: First cases of Q fever in Poland. Polski Tygodnik Lekarski 11(46): 1950-1955

Yoshimoto, T.; Takahashi, E.; Yamashita, S.; Ohara, K.; Niimi, K., 2017: Larger cages with housing unit environment enrichment improve the welfare of marmosets. The provision of adequate space for laboratory animals is essential not only for good welfare but accurate studies. For example, housing conditions for primates used in biomedical research may negatively affect welfare and thus the reliability of...