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Immunolocalization of a pan - specific sodium channel polyclonal antibody at the photoreceptor synaptic ribbons of the goldfish retina


, : Immunolocalization of a pan - specific sodium channel polyclonal antibody at the photoreceptor synaptic ribbons of the goldfish retina. Society for Neuroscience Abstract Viewer & Itinerary Planner : Abstract No 817 6

Voltage dependent sodium channels are fundamental components of spiking neuronal signal propagation. They also play a role in non-spiking neurons, as characterized in retinal bipolar neurons (Zenisek et al.,'01). Utilizing a commercially available (Upstate) pan-specific sodium channel (PanNaCh) antibody against the highly conserved intracellular III-IV loop, immunocytochemical evidence confirms the presence of PanNaCh immunoreactivity (PanNaCh-IR) in goldfish retina. In Western blots, the PanNaCh antibody recognizes a single apprx220 kDa band in rat brain. In goldfish brain, the antibody recognizes two discrete bands, one at apprx220 kDa and one at apprx110 kDa. At the light level this antibody punctately labels membrane sections of cone bipolar cell bodies, fibers in the IPL, and unlike other previously published pan-specific sodium channel antibodies, the membrane of photoreceptor terminals. A pre-embed gold substituted silver intensification reaction was performed in order to determine the subcellular localization of the PanNaCh-IR in photoreceptors. At the electron microscopic level immunoreactivity was associated with rod and cone synaptic ribbons. Current morphological and physiological data suggest that synaptic ribbons function in recruiting vesicles for release at the active zone. However, little is know of ribbon structural biochemistry. These data suggest a metabolic role for synaptic ribbons.

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