geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Increased sensitivity of myeloid leukemia cell lines: potential of lovastatin as bone-marrow-purging agent


, : Increased sensitivity of myeloid leukemia cell lines: potential of lovastatin as bone-marrow-purging agent. Acta Haematologica 104(2-3): 72-79

Lovastatin reduces the isoprenylation of p21ras via suppression of mevalonic acid generation. Lovastatin has been shown to reduce tumor cell proliferation in a dose-dependent manner. Here, the potential of lovastatin for purging leukemia cells from bone marrow was investigated using the myeloblastic cell lines K562 and KG-1 as a model system, derived from an erythroleukemia and an acute myelogenous leukemia, respectively. Optimal purging conditions were determined using an MTT proliferation and a leukemia colony assay. Elimination of leukemia cells was time- and dose-dependent. Depletion of K562 was 2.5 logs for 100 microM of lovastatin at 72 h of incubation. Compared to another purging agent, 100 microg/ml mafosfamide had an activity comparable to 100 mM lovastatin. Interestingly, KG-1 acute myelogenous leukemia cells were even more sensitive to lovastatin than K562 cells. In clonogenic assays, 100 microM of lovastatin resulted in a 3- to 4-log reduction of K562 colonies. Lovastatin had a progressive effect on normal hematopoietic progenitor cells. At a concentration of 100 microM of lovastatin, CFU-GM colonies were reduced by 1-2 logs. In conclusion, a differential effect on leukemia and normal progenitor cells could be detected in a clonogenic assay. These results suggest that lovastatin deserves further study as an agent for ex vivo marrow purging.

US$19.90

PMID: 11154978

DOI: 39755


Other references

Aatola, M.; Armstrong, E.; Teerenhovi, L.; Borgstroem, G., H., 1992: Clinical significance of the del(20Q) chromosome in hematologic disorders. Patients with hematologic neoplasias often have chromosomal aberrations in the cells of their bone marrow or unstimulated blood. One recurrent abnormality is a deletion of the long arm of chromosome 20, primarily described in polycythemia vera, bu...

Koch, 1994: Temperature dependence of correlation functions in the spacelike direction in hot QCD. Physical Review. D, Particles and Fields 49(11): 6063-6071

Sawyer E.W., 1991: Disequilibrium melting and the difference between the trace element contents of migmatite leucosomes and granites. Record - Bureau of Mineral Resources, Geology and Geophysics Pages 91

D.P.ol, G.; Rivera Pirela, S.; Torres, T.; Perez, M.; Garcia, A.; Castejon, O.; Rojas, N., 2004: Prevalence of bovine brucellosis using the competitive ELISA test in La Canada de Urdaneta Municipality, Zulia State, Venezuela. A seroepidemiological study was made in all sectors (1 to 10) of La Canada de Urdaneta municipality of Zulia state in 1999 to determine the prevalence of bovine brucellosis and evaluate the risk factors which promote the presence of the disease. A...

Edwards, D.L.; Keogh, J.S.; Knowles, L.L., 2012: Effects of vicariant barriers, habitat stability, population isolation and environmental features on species divergence in the south-western Australian coastal reptile community. Identifying explicit hypotheses regarding the factors determining genetic structuring within species can be difficult, especially in species distributed in historically dynamic regions. To contend with these challenges, we use a framework that com...

Cayouette, M.; Raff, M., 2002: Asymmetric cell division and Numb segregation in the developing mammalian retina. Developmental Biology 247(2): 499, July 15

Avignon, A.; du Cailar, G.; Ribstein, J.; Monnier, L.; Mimran, A., 1997: Determinants of the left ventricular mass in obese patients. Influence of lean body mass. Systolic blood pressure and body mass index (BMI) are the main determinants of the left ventricular mass (LVM). The mechanism of this cardiac hypertrophy in the obese individual is multifactorial and involves hemodynamic as well as metabolic facto...

Ohno, S.; Ikeda, Y.; Onitsuka, T.; Nakajima, S.; Haraoka, J., 2004: Bilateral chronic subdural hematoma in a young adult mimicking subarachnoid hemorrhage. A 33-year-old man was admitted to our hospital with a sudden severe headache five days after the onset. CT scan showed a slight high-density area in the basal cistern, mimicking subarachnoid hemorrhage (SAH), and diffuse brain swelling. However, c...

Woychik R.P.; Beatty B.R.; Mckinney W.L.; Andreadis D.A.; Chang A.J.; Barker P.E., 1990: Insertional mutagenesis in transgenic mice. Increasing numbers of transgenic mouse lines have resulted in several dozens of mutants created by insertional mutagenesis. The advantages of different vector systems and the problems associated with the analysis of mutations and the cloning of th...

Singano, V.; Amberbir, A.; Garone, D.; Kandionamaso, C.; Msonko, J.; van Lettow, M.; Kalima, K.; Mataka, Y.; Kawalazira, G.; Mateyu, G.; Kwekwesa, A.; Matengeni, A.; van Oosterhout, J.J., 2017: The burden of gynecomastia among men on antiretroviral therapy in Zomba, Malawi. Many Africans who are on life-saving ART face challenges from a variety of toxicities. After the introduction of a standardized first-line efavirenz-containing ART regimen, reports of gynecomastia appeared in Malawian popular media, however data o...