+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Is use of the hydrophobic moment a sound basis for predicting the structure-function relationships of membrane interactive alpha-helices?

, : Is use of the hydrophobic moment a sound basis for predicting the structure-function relationships of membrane interactive alpha-helices?. Current Protein & Peptide Science 4(5): 357-366

Amphiphilic alpha-helices play a fundamental role in protein membrane association and show a segregation of polar and apolar amino acid residues. Based on correlations between amphiphilic properties and biological function, a number of theoretical approaches have been developed, which quantify alpha-helix amphiphilicity and then attempt to assign function. The most commonly used measure of amphiphilicity is the hydrophobic moment, < microH >, which, when used in conjunction with an alpha-helix's mean hydrophobicity, < H >, has been used to classify membrane interactive amphiphilic alpha-helices as either surface active or transmembrane. Here, the predictive efficacy of plot methodology is reviewed by examining published data, which compare the function of known membrane interactive amphiphilic alpha-helices to that assigned by this methodology. The results of this review are discussed in relation to the reliability of < microH > as a quantifier of alpha-helical amphiphilicity, and the ability of < microH > and < H > to describe alpha-helical structure / function relationships. It is concluded that hydrophobic moment plot methodology is not a generally reliable predictor of alpha-helical structure / function relationships. It appears that the inefficacy of plot methodology is primarily due to the inability of the plot diagram to accommodate the heterogeneity of the alpha-helical classes it attempts to define. However, the predictive efficacy of the methodology appears to be improved if other alpha-helical parameters are also considered when assigning alpha-helical function. It is suggested that the conventional methodology should be seen only as an indicator for the assignation of structure / function relationships, providing a guide to future experimental investigations.


PMID: 14529529

Other references

Barra, D.; Simmaco, M., 1995: Amphibian skin: a promising resource for antimicrobial peptides. Amphibian skin is a rich source of biologically active compounds that are assumed to have diverse physiological and defence functions. In addition to the range of pharmacologically active peptides present, some of which have mammalian homologues,...

Gallo, L.; Vignale, L., 1991: Training course for voluntary relief assistants: analysis of the experience at the International Red Cross of Massa-Carrara. Minerva Anestesiologica 57(12): 1747-1750

Costello, C.B.; Jasani, B.; Kumar, S., 1991: Tamm-Horsfall protein in human renal tumours. Tamm-Horsfall Protein (THP) is found to be localised in the ascending loop of Henle and the distal tubules of normal and foetal kidneys. We have used an anti-THP monoclonal antibody (D2) to stain foetal, normal and kidney tumours. The dinitropheny...

Avrameas, S., 1968: Detection of antibodies and antigens by means of enzymes. Bulletin de la Societe de Chimie Biologique 50(5): 1169-1178

Schild L.; Aronson P.S.; Giebisch G., 1990: Effects of apical membrane chloride formate exchange on cell volume in rabbit proximal tubule. We used real-time recordings of cell volume changes to test for the role of the Cl--formate exchanger in mediating NaCl entry across the apical membrane of rabbits proximal tubule cells. In the absence of extracellular Cl-, 0.5 and 5 mM formate in...

Chitambar, C.R.; Wereley, J.P., 1995: Effect of hydroxyurea on cellular iron metabolism in human leukemic CCRF-CEM cells: changes in iron uptake and the regulation of transferrin receptor and ferritin gene expression following inhibition of DNA synthesis. Hydroxyurea inhibits cellular proliferation through action on ribonucleotide reductase, an iron-dependent enzyme responsible for the synthesis of deoxyribonucleotides. Whereas previous investigations have examined the interaction of hydroxyurea wi...

Rácz, P.; Tenner, K.; Szivessy, K., 1971: Morphogenesis of keratoconjunctivitis shigellosa. Orvosi Hetilap 112(42): 2517-2520

Filipponi, A.; Dojmi D.D.lupis, G., 1964: Sulla biologia e capacita riproduttiva di Macrocheles penicillatus Berlese (Acari : Mesostigmata) in condizioni sperimentali di laboratorio. Rivista di Parassitologia, 25: 93-113

Zhang, Y.; Zhang, P.; West, C.M., 1999: A linking function for the cellulose-binding protein SP85 in the spore coat of Dictyostelium discoideum. SP85 is a multidomain protein of the Dictyostelium spore coat whose C-terminal region binds cellulose in vitro. To map domains critical for localizing SP85 and for binding to other proteins in vivo, its N- and C-terminal regions, and a hybrid fusi...

Ju, W.; Myung, S-Kwon.; Kim, Y.; Choi, H.Jae.; Kim, S.Cheol., 2009: Comparison of laparoscopy and laparotomy for management of endometrial carcinoma: a meta-analysis. This meta-analysis was performed to investigate the outcome of laparoscopic surgery for endometrial carcinoma compared with laparotomy. We searched the MEDLINE (PubMed), EMBASE, and Cochrane Review databases in September 2007. Three independent ev...