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Microcirculatory responses to perfusion of isolated cremaster muscle flaps with preservation solutions

, : Microcirculatory responses to perfusion of isolated cremaster muscle flaps with preservation solutions. FASEB Journal 15(4): A36, March 7

Introduction Histidine-Tryptophan-Ketoglutarate (HTK), an intracellular type low viscosity preservation solution, is thought to have beneficial effects on rat skeletal muscle preservation. The benefits, however, may be countered by ischemia-reperfusion injury associated with HTK perfusion. The aim of the study was to determine whether HTK perfusion and subsequent blood reperfusion caused microvascular disturbances. Methods An isolated rat cremaster muscle flap was employed, which allowed direct in vivo inspection and measurement of microcirculatory parameters, including leukocyte-endothelium interactions, capillary perfusion, and arteriole diameters. The cremaster muscle was perfused for 10 minutes with HTK solution (20degreeC) via the femoral artery with the iliac artery occluded. Then the cremaster muscle was subjected to 4 hours of ischemia and 2 hours of reperfusion. Baseline microvascular measurements were recorded prior to HTK perfusion, with subsequent measurements repeated at 0, 1 and 2 hours after restoration of blood flow. Results During perfusion with HTK blood was quickly cleared from the microcirculation. No differences in leukocyte behavior were observed compared to baseline or the control group. The capillary perfusion was temporarily decreased after ischemia with return to normal during the 2 hours of reperfusion. No differences in any of the microvascular measurements were found between HTK perfused and non-HTK perfused groups. Conclusions We introduce a vascular isolated rat cremaster muscle model as a useful preparation to study the effects of perfusion solutions on the microcirculation after 4 hours of ischemia and 2 hours of reperfusion. HTK showed no detrimental effects on the microcirculation compared to control. However, a beneficial effect of HTK perfusion at 20degreeC was not evident. Studies with cold HTK perfusion may still show preservative effects on the microcirculation, and further improve results of muscle preservation.


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