geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Molecular and cDNA microarray analysis of steroid - induced muscle degeneration during insect metamorphosis


, : Molecular and cDNA microarray analysis of steroid - induced muscle degeneration during insect metamorphosis. Society for Neuroscience Abstract Viewer & Itinerary Planner : Abstract No 825 4

During metamorphosis in the hawkmoth, Manduca sexta, segmental homologs of the accessory planta retractor muscle (APRM) undergo programmed cell death (PCD) in a segment-specific pattern. The PCD of APRMs is triggered directly and cell-autonomously by the steroid hormone, 20-hydroxyecdysone (20E). To seek genes involved in intrinsic segmental identity, 20E signaling and muscle degeneration, we performed suppressive subtractive hybridization (SSH) on mRNA from APRMs in abdominal segments A3 and A6; APRM(6)s undergo PCD while APRM(3)s survive during the larval-pupal transformation. mRNA was obtained on the first day of the prepupal peak of 20E. We generated a library of approximately 2000 clones, some of which are likely redundant. These cDNAs, plus other candidate cDNAs, were spotted on microarrays and hybridizations are in progress to study gene expression in APRMs during prepupal 20E signaling. Five hundred of the clones were sequenced and, based on comparison with Drosophila and other species, many encode potentially interesting regulators of development. Concurrently, we examined the expression of genes in the Bithorax Complex (Bx-C) in APRMs, using real-time PCR. These genes regulate axial patterning of the insect abdomen. Real-time PCR data suggest that Bx-C genes are expressed in APRMs in a segmental pattern similar to that seen in fly embryos. The Bx-C genes appear to be expressed at very low levels in larvae but strongly upregulated by the prepupal peak of 20E. Potentially, the expression of axial patterning genes of the embryonic stage is recapitulated during metamorphosis to direct the segmental fates of individual cells in response to hormonal cues.

US$29.90


Other references

Simm K., 1985: Integration of scientific investigations for the protection of water bodies. Eesti NSV Teaduste Akadeemia Toimetised Bioloogia 34(2): 85-93

Lenggenhager, K., 1964: Degeneration And Calcification Of The Tendon Of The Supraspinal Muscle. Etiology And Treatment. Schweizerische Medizinische Wochenschrift 94: 1188-1193

McCreary, A.; Ghosh, R.; Amani, M.; Wang, J.; Duerloo, K-Alexander.N.; Sharma, A.; Jarvis, K.; Reed, E.J.; Dongare, A.M.; Banerjee, S.K.; Terrones, M.; Namburu, R.R.; Dubey, M., 2016: Effects of Uniaxial and Biaxial Strain on Few-Layered Terrace Structures of MoS₂ Grown by Vapor Transport. One of the most fascinating properties of molybdenum disulfide (MoS2) is its ability to be subjected to large amounts of strain without experiencing degradation. The potential of MoS2 mono- and few-layers in electronics, optoelectronics, and flexi...

Oja, T.; Laarmann, H., 2002: Comparative study of the ploidy series Bromus sterilis, B. diandrus and B. rigidus (Poaceae) based on chromosome numbers, morphology and isozymes. Chromosome numbers, morphological characters and isoenzymes of seven enzymes were studied to assess relationships between species of the Bromus diandrus-rigidus polyploid complex and the closely related species, B. sterilis. The four different cyt...

Mikode, A.D.; Stokes, M.R.; Barton, B.A., 1996: Improving agricultura resource management in Benin. Journal of Animal Science 74(SUPPL 1): 100

Rodrigues, D.Bertulucci.Rocha.; Correia, D.; Marra, Mônica.Dias.; Giraldo, L.Eduardo.Ramirez.; Lages-Silva, E.; Silva-Vergara, M.León.; Barata, C.Hueb.; Rodrigues Junior, V., 2006: Cytokine serum levels in patients infected by human immunodeficiency virus with and without Trypanosoma cruzi coinfection. This study assessed the number of CD4 T lymphocytes, the parasitemia and serum levels of interferon gamma (IFN-gamma), tumor necrosis factor alpha (TNF-alpha), interleukin-1 (IL-1), IL-4 and IL-10 of patients infected by human immunodeficiency vir...

Stratmann H.G.; Younis L., 1992: Prognostic value of dipyridamole thallium 201 imaging in patients with stable angina pectoris. Clinical Research 40(2): 405A

Anonymous, 1998: Rural travel and tourism focus of national conference. Rural development news 22(3): 8-9

Reyment R.A., 1983: Internal structure of bolivina and afrobolivina. Structural elements of the type species of Bolivina, B. plicata d'Orbigny, are compared and contrasted with those of Afrobolivina afra Reyment. The cameral folds of Bolivina (which are not retral processes) are integral parts of the external...

Fenton, M.; Sanders, C., 2002: Building better bone. Positive Living 11(5): 47-48