geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Molecular typing of clinical isolates of Stenotrophomonas maltophilia strains by pulsed-field gel electrophoresis and random primer PCR fingerprinting


, : Molecular typing of clinical isolates of Stenotrophomonas maltophilia strains by pulsed-field gel electrophoresis and random primer PCR fingerprinting. Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 43: 365

Background: World wide increasing numbers of S. maltophilia strains are isolated in specimens from patients with nosocomial infections. In order to detect possible nosocomial outbreaks, the profiles of the clinical strains of S. maltophilia must be investigated to asses possible clonality. Methods: During a 1-year study period, 127 strains of S. maltophilia were isolated from different specimens of 77 patients at a 1,500 bed major tertiary care teaching university hospital. The strains were identified by commercial available API system and genotyped by pulsed-field gel electrophoresis (PFGE) and random primer PCR fingerprinting. Results: Only few isolates from different specimens of the same patient were clonally identical. In other patients the strains of the same patient isolated from different specimens were clonally different. However, comparing to PFGE the PCR based method shows similar results but is easier and faster to perform. Conclusions: Due to the wide variation of the clonally profiles of the investigated S. maltophilia strains shown by PFGE as well as PCR typing, our results suggest that there was no outbreak situation of S. maltophilia strains with identical profiles at our hospital.

US$29.90


Other references

Ng, W.K.; Taylor, N.F.; Hughes, I.A.; Taylor, J.; Ransley, P.G.; Grant, D.B., 1990: 5 alpha-reductase deficiency without hypospadias. A boy aged 4 with penoscrotal hypospadias and his brother aged 12 with micropenis had typical changes of homozygous 5 alpha-reductase deficiency. After three injections of chorionic gonadotrophin there was a trivial rise in plasma dihydrotestoster...

Jacobs, A.D.; Champlin, R.E.; Golde, D.W., 1985: Recombinant alpha-2-interferon for hairy cell leukemia. Twenty-two patients with hairy cell leukemia were treated with biosynthetic (recombinant) alpha-2-interferon in an open-label, single-arm efficacy study. Patients received 2 X 10(6) U/m2 recombinant alpha-2-interferon three times weekly. Therapy w...

Scheerpeltz, O., 1957: Neue Arten der Untergattung Sphenoma Mannerh. der Gattung Oxypoda Mannerh. mit einer neuen Bestimmungstabelle der palaarktischen Arten dieser Untergattung (Col. Staphylinidae). Norsk Entomologisk Tidsskrift, 10: 136-152

Johnson, M.H.; Munakata, Y., 2005: Processes of change in brain and cognitive development. We review recent advances in the understanding of the mechanisms of change that underlie cognitive development. We begin by describing error-driven, self-organizing and constructivist learning systems. These powerful mechanisms can be constrained...

Stewart, C.E.; Nijmeh, H.S.; Brightling, C.E.; Sayers, I., 2012: uPAR regulates bronchial epithelial repair in vitro and is elevated in asthmatic epithelium. The asthma-associated gene urokinase plasminogen activator receptor (uPAR) may be involved in epithelial repair and airway remodelling. These processes are not adequately targeted by existing asthma therapies. A fuller understanding of the pathway...

Raghuramulu, Y.; Thimmaraju, K.R., 1998: Early observations on graft compatibility between commercial arabica coffee cultivars and desirable rootstocks. Compatibility between two arabica coffee (Coffea arabica L.) cultivars viz., Cauvery (Catimor) and S.795 and six different rootstocks of coffee was investigated as a step towards identifying suitable rootstocks for arabica types susceptible to moi...

Blodget, BG.; Lyons, PJ., 1988: The recolonization of Massachusetts by the common loon (Gavia immer). Unknown

Gilleard, J.Stuart., 2006: Understanding anthelmintic resistance: the need for genomics and genetics. Anthelmintic resistance is a major problem for the control of many parasitic nematode species and has become a major constraint to livestock production in many parts of the world. In spite of its increasing importance, there is still a poor unders...

Herschbach, P.; Duran, G.; Waadt, S.; Zettler, A.; Amm, C.; Marten-Mittag, B., 1997: Psychometric properties of the Questionnaire on Stress in Patients with Diabetes--Revised (QSD-R). This study reports the psychometric properties of a 45-item diabetes-specific questionnaire, the Questionnaire on Stress in Patients with Diabetes--Revised (QSD-R), a modified and shortened version of the QSD (G. Duran, P. Herschbach, S. Waadt, A....

Dokmeci, M.R.; Khademhosseini, A., 2011: Preface to Special Topic: Microfluidics in cell biology and tissue engineering. In this special issue of Biomicrofluidics, a wide variety of applications of microfluidics to tissue engineering and cell biology are presented. The articles illustrate the benefits of using microfluidics for controlling the cellular environment i...