geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

NADH and collagen as quantitative biomarkers for endoscopic detection of pre-cancers


, : NADH and collagen as quantitative biomarkers for endoscopic detection of pre-cancers. FASEB Journal 15(4): A250, March 7

During the development of neoplasia, epithelial tissues undergo biochemical and structural changes that can manifest in tissue fluorescence. While there have been some reports on different fluorescence characteristics between normal and dysplastic tissues, it has been difficult to identify the origins of these changes. We have previously described methods for recovery of intrinsic fluorescence that are prerequisite for extracting the spectroscopic signatures of major tissue fluorophores. Here, we present evidence that NADH and collagen spectra can describe the bulk intrinsic tissue fluorescence in the esophagus and the uterine cervix. Using an optical probe coupled to a fast excitation emission matrix (FastEEM) instrument, sets of fluorescence spectra at 11 different excitation wavelengths and a white light reflectance spectrum were endoscopically acquired in 1 second from patients with esophageal varices prior to, and at 2, 3, 5 and 15 minutes following ligation of varices. Changes in intrinsic fluorescence EEMs during hypoxia were shown to be consistent with a two-component system with contributions from NADH and collagen. To determine whether NADH and collagen contributions to the overall intrinsic tissue fluorescence can be used as biomarkers for detection of dysplasia, we analyzed spectra acquired colposcopically in patients with cervical lesions and endoscopically in patients with Barrett's esophagus (BE). A significant decrease in the relative contribution of collagen to NADH characterized squamous metaplasia and high-grade intraepithelial neoplasia compared to normal ectocervix. Similarly, in patients with BE, the ratio of NADH to collagen increased significantly during the development of high-grade dysplasia. These data for the first time present evidence for the presence of detectable levels of NADH fluorescence in epithelial tissues, and demonstrate that NADH and collagen may be used as quantitative fluorescence biomarkers for in vivo detection of dysplasia in cervix and esophagus.

US$29.90


Other references

Harten, A.M. van; Bouter, H., 1974: The 'Joergensen' method, does it produce adventitious sprouts from potato?. During work on adventitious sprout production from apex layer L1 tissue, sprouts were produced at the base of plantlets severed from tubers using the Joergensen method on red-splashed stable monectochimeric clones B165 and EM 52. The colour of tub...

Guilleminault, C.; Eldridge, F.; Dement, W.C., 1972: Insomnia, narcolepsy, and sleep apneas. Bulletin de Physio-Pathologie Respiratoire 8(5): 1127-1138

He, Q.; Li, H.; Rui, Y.; Liu, L.; He, B.; Shi, Y.; Su, X., 2017: Pentraxin 3 gene polymorphisms and pulmonary aspergillosis in COPD patients. Pentraxin 3 (PTX3) plays an important role in resistance to Aspergillus infections. Previous studies have suggested that PTX3 polymorphisms influence the risk of invasive aspergillosis among transplantation recipients. This study was to investigat...

Yamanoue, M.; Ueda, S.; Ohashi, A., 2003: The N-Terminal Sequence of Paratropomyosin Binding Fragments from b-Connectin. Bioscience, Biotechnology, and Biochemistry 67(3): 3-9

Adnan, T.; Elif, A.A.; Ayşe, K.; Gülnaz, A., 2005: Clonidine as an adjuvant for lidocaine in axillary brachial plexus block in patients with chronic renal failure. Clonidine is often used as an adjuvant for local anaesthetics in regional anaesthesia; however, its effects on axillary block in chronic renal failure patients have not been investigated. These effects were the focus of this study.Twenty-eight adu...

Schaffner, K.F., 1969: Chemical systems and chemical evolution: the philosophy of molecular biology. American Scientist 57(4): 410-420

Minoda, H.; Okabe, T.; Iijima, H., 2012: Contrast enhancement in the phase plate transmission electron microscopy using an objective lens with a long focal length. A new optical condition using an objective lens (OL) of a long focal length (objective mini lens: OM) was tested to enhance image contrast in phase plate transmission electron microscopy (P-TEM). A phase plate was set on the selected area aperture...

Peixoto, M.Manuela.; Nobre, P., 2015: Dysfunctional sexual beliefs: a comparative study of heterosexual men and women, gay men, and lesbian women with and without sexual problems. Conservative and dysfunctional sexual beliefs are commonly associated with sexual problems among heterosexual men and women. However, little is known about the role of sexual beliefs in sexual problems in gay men and lesbians. The present study ai...

Sobin, C.; Prudic, J.; Devanand, D.P.; Nobler, M.S.; Sackeim, H.A., 1996: Who responds to electroconvulsive therapy? A comparison of effective and ineffective forms of treatment. Background: It has been reported that real ECT is more effective than simulated treatment among depressed patients with delusions and/or retardation, and that ECT is not effective among depressed patients who lack these features. Method: In two ra...

Vanneste, J.L., 1996: Honey bees and epiphytic bacteria to control fire blight, a bacterial disease of apple and pear. A review of current literature indicated that incidence of fire blight (Erwinia amylovora) can be reduced by spraying apple and pear flowers with some strains of E. herbicola or Pseudomonas fluorescens. Most of these beneficial bacteria were selec...