+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content


, : P38 MAP KINASE AND CASPASE - 3 MEDIATE Fe2+ - INDUCED NEURONAL DEATH IN CORTICAL CELL CULTURES. Society for Neuroscience Abstract Viewer & Itinerary Planner : Abstract No 292 17

We examined the possibility that p38 MAP kinase and CASPASE-3, the main mediators of cell death, would mediate free radical-induced neuronal cell NECROSIS. As previously reported, cortical cell cultures exposed to 30 uM Fe2+ underwent widespread neuronal cell necrosis evident by marked cell body swelling over the next 24 hr. The active form of p38 MAP kinase was observed within 4 hr following exposure of cortical cultures to Fe2+. Inclusion of PD169316, a selective inhibitor of p38 MAP kinase, attenuated Fe2+-induced neuronal cell necrosis. We next examined if CASPASE-3 would also mediate Fe2+ neurotoxocity as a downstream signal of p38 MAP kinase. The active fragment of CASPASE-3 was observed within 12 hr following exposure of cortical cell cultures to Fe2+. Fe2+-induced neuronal death was attenuated by inclusion of z-VAD-fmk,a broad-spectrum inhibitor of caspases. However, inclusion of PD169316 did not block Fe2+-induced activation of CASPASE-3, suggesting that p38 MAP kinase and CASPASE-3 separately mediate Fe2+-induced free radical neurotoxicity. In support of this, concurrent administration of PD169316 and z-VAD-fmk synergistically prevented Fe2+ neurotoxicity. The present study suggests that the well-known mediators of APOPTOSIS , p38 MAP kinase and CASPASE-3 also mediate Fe2+-induced neuronal cell necrosis possibly through separate routes.


Other references

Giladi I., 1979: The role of ethylene in abscisic acid induced callus formation in citrus sinensis cultivar shamouti bud cultures. Plant Physiology (rockville)suppl): 92

Al'perin P.M.; Mikhailova L.I.; Solov'eva T.I.; Sharova Y.A., 1974: Changes in contractile function indices of the myo cardium and the blood coagulation system under the effect of hemo transfusions in patients with cardio sclerosis and anemia. Problemy Gematologii i Perelivaniya Krovi 19(2): 8-15

Biswas, S., 1970: A preliminary survey of gharial in the Kosi River,. Indian Forester, 96: 705-710

Kramer J.H.; Misik V.; Weglicki W.B., 1992: Magnesium deficiency enhances free radical production in post ischemic rat hearts. FASEB Journal 6(4): A1342

Piepoli, A.; Santoro, R.; Cristofaro, G.; Siena, D.; Accadia, L.; Gennarelli, M.; Andriulli, A.; Peltomaki, P.; D.L.C.apelle, A., 1995: Linkage analysis in families with Lynch II syndrome identifies healthy subjects at risk. Gastroenterology 108(4 SUPPL ): A525

Midcap, J.; Johnson, C., 1982: Osmocote benefits cuttings in mist beds. Southern florist and nurseryman6, 95(18): 59-62

Freiman, Z., 1992: The Tenderloin AIDS Resource Center. Progressive Architecture 73(5): 136-137

Valeins, H.; Merle, M.; Labouesse, J., 1992: Pre-steady state study of beta-adrenergic and purinergic receptor interaction in C6 cell membranes: undelayed balance between positive and negative coupling to adenylyl cyclase. Interactions between beta-adrenergic and ADP purinergic receptors in C6 glioma cell membrane preparations were investigated under steady state and then pre-steady state conditions of adenylyl cyclase (EC activity, in order to determine ho...

Coelho, R.R., 1966: Nota sobre a reproducao das lagostas Panulirus argus (Latreille) e P. laevicauda (Latreille) (Decapoda: Paluridae) no literal do estado de Pernambuco. Trab Oceanogr., Univ. do Recife: 61-67

Shalaby A.M.; Ramadan A.A.; Abdel Ghaffar H.; Abdelsaboor S., 1985: Sexing of living larvae and pupae of culex pipiens molestus diptera culicidae. Sexing of living Culex pipiens molestus larvae and pupae was carried out on the basis of two parameters. The first were the measurements of the head, the thorax and the first, eighth and anal abdominal segments in fourth instar larvae; the cephalo...