geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

P53 transcription activation and BH3 domain - only pro - apoptotic gene expression in neuronal apoptosis after focal cerebral ischemia


, : P53 transcription activation and BH3 domain - only pro - apoptotic gene expression in neuronal apoptosis after focal cerebral ischemia. Society for Neuroscience Abstract Viewer & Itinerary Planner : Abstract No 739 12

Transcription factor p53 has been implicated in the trigger of apoptotic cell death by regulating expression of its downstream target genes. p53-upregulated modulator of apoptosis (PUMA) and Noxa encode a BH3 domain but not other BH domains and undergo BH3 motif-dependent localization to mitochondria and interact with Bcl-2 family proteins, resulting in the activation of mitochondria-dependent apoptotic signal cascade. The present series of studies were aimed to investigate the involvement of p53 and their downstream pro-apoptotic genes, PUMA and Noxa, in neuronal apoptosis after cerebral ischemia. Transient focal cerebral ischemia (tFCI) was induced by middle cerebral artery occlusion (MCAO) using intraluminal filament technique. The amount of PUMA and Noxa gene expression was increased in the ipsilateral cerebral cortex measured by semi-quantitative RT-PCR and western blotting. Puma underwent mitochondrial translocalization and physically interacted with anti-apoptotic Bcl-2 family member, Bcl-xL, resulting in the activation of caspase-3. Furthermore, PUMA and Noxa were significantly expressed in apoptotic neuronal cells, observed by Terminal deoxynucleotydyl transferase (TdT)-mediated dUTP-biotin nick-end-labeling method (TUNEL) staining and coimmunohistochemisty with neuronal marker, NeuN. Activation of p53 DNA binding preceded the peak expression of PUMA and Noxa. Although further evaluation is necessary to elucidate the regulation of BH3-only proteins after brain ischemia, our results indicate that the BH3 domain-only pro-apoptotic proteins, PUMA and Noxa, are involved in ischemia-induced neuronal apoptosis through mitochondria-dependent apoptotic pathway. Transcriptional regulation may play an important role in BH3 domain-only protein-mediated neuronal apoptosis.

US$29.90


Other references

da Silva, A.Zaccaron.; Zanette, J.; Fernando Ferreira, J.; Guzenski, Jão.; Marques, M.Risoleta.Freire.; Bainy, A.Celso.Dias., 2005: Effects of salinity on biomarker responses in Crassostrea rhizophorae (Mollusca, Bivalvia) exposed to diesel oil. Crassostrea rhizophorae is a euryhaline oyster that inhabits mangrove areas, which are widely distributed along the Brazilian coast. The aim of this study was to investigate the effects of salinity (9, 15, 25, and 35ppt) on the activities of gluta...

Isaka, Y.; Takabatake, Y.; Imai, E., 2006: RNAi gene therapy for kidney disease. Nihon Rinsho. Japanese Journal of Clinical Medicine 64 Suppl 2: 659-661

Alhabib, S.; Aldraimly, M.; Alfarhan, A., 2016: An evolving role of clinical pharmacists in managing diabetes: Evidence from the literature. Diabetes mellitus is a rapidly growing disease world-wide that is estimated to be present in 6.6% of the international population and projected to be increased by 7.8% in 2030. Treating diabetic patients is multifaceted in all aspects and they req...

Heyer, W.R.nald; Garcia Lopez, J.M.; Cardoso, A.J., 1996: Advertisement call variation in the Leptodactylus mystaceus species complex with a description of a new sibling species. Whereas morphological analysis of populations recognized as Leptodactylus mystaceus indicates there is one species with modest geographic variation, analysis of advertisement calls indicates there are at least two or perhaps three species involved...

Lee, M.S.; Thompson, J.Kevin., 2016: Exploring enhanced menu labels' influence on fast food selections and exercise-related attitudes, perceptions, and intentions. Labeling restaurant menus with calorie counts is a popular public health intervention, but research shows these labels have small, inconsistent effects on behavior. Supplementing calorie counts with physical activity equivalents may produce strong...

Moussa, M.; Goldberg, S.Nahum.; Kumar, G.; Levchenko, T.; Torchilin, V.; Ahmed, M., 2016: Effect of thermal dose on heat shock protein expression after radio-frequency ablation with and without adjuvant nanoparticle chemotherapies. The aim of this study was to evaluate the effect of different radio-frequency ablation (RFA) thermal doses on coagulation and heat shock protein (HSP) response with and without adjuvant nanotherapies. First, Fischer rats were assigned to nine diff...

Hsu, P-Feng.; Cheng, H-Min.; Wu, C-Hsueh.; Sung, S-Hsien.; Chuang, S-Yuan.; Lakatta, E.G.; Yin, F.C.P.; Chou, P.; Chen, C-Huan., 2016: High Short-Term Blood Pressure Variability Predicts Long-Term Cardiovascular Mortality in Untreated Hypertensives But Not in Normotensives. The prognostic value of the short-term blood pressure variability (BPV) from the 24-hour ambulatory blood pressure monitoring (ABPM) remains controversial. The present study aimed to investigate the long-term prognostic value of a high BPV in norm...

Notman, M.T.; Zinberg, N.E., 1969: Functions of a short-term therapy program. Problems of the maintenance of quality. Archives of General Psychiatry 20(4): 403-407

Booth, K.A.; Barkley, G.M., 1995: Pea epicotyl lateral growth Regulation by calcium and low temperature. Plant Physiology (Rockville) 108(2 SUPPL ): 81

Ucko, M.; Colorni, A., 2005: Mycobacterium marinum infections in fish and humans in Israel. Israeli Mycobacterium marinum isolates from humans and fish were compared by direct sequencing of the 16S rRNA and hsp65 genes, restriction mapping, and amplified fragment length polymorphism analysis. Significant molecular differences separated a...