geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Post-thaw losses of progenitor and T cells from peripheral blood stem cells cryopreserved in a pentastarch solution do not increase for up to 2 hours after thawing


, : Post-thaw losses of progenitor and T cells from peripheral blood stem cells cryopreserved in a pentastarch solution do not increase for up to 2 hours after thawing. Blood 96(11 Part 1): 520a, November 16

Current practice guidelines dictate that thawing and infusion of cryopreserved PBSC be carried out as rapidly as possible. To support our institutional policies for thawing in the laboratory prior to transporting PBSC to clinical care units and to allow for unforeseen infusion delays, we studied the effect of extended room temperature (RT) storage on thawed PBSC. PBSC were collected on the Fenwal CS3000 from 12 G-CSF mobilized normal donors (12 L volume processed). Unseparated PBSC were cryopreserved in Cryocyte bags (Nexell) using a controlled rate freezer in 5% DMSO/6% pentastarch/4% HSA, placed in an overwrap bag, and stored in liquid nitrogen for 2 weeks. All 12 products were thawed rapidly in a 37degreeC water bath, diluted 1:4 with heparinized Plasmalyte A (Baxter), and held at RT for 120 min. Samples were obtained before cryopreservation and at 30 and 120 min after thawing, for cell counting, flow cytometric phenotyping and viability, and clonogenic assays. Values for content of total nucleated cells (TNC), CD34+ cells, CD3+ cells, and CFU-GM in the table represent mean (S.D.) of samples from 12 bags, each of which contained 1/4 of the initial product. Values for CD34+ and CD3+ cells represent viable (7AAD-neg) cells that express those markers. From pre-freeze to 30 min. post-thaw, expected losses occurred in % viability of the overall product (98% to 78%) and TNC (11.6 to 8.9 X 109). Loss of CD34+ cells was minimal (110 to 98 X 106; 89% recovery), but loss of CD3+ cells was substantial (4.0 to 1.6 X 109, 40% recovery). No significant further losses occurred between 30 min and 120 min for any of the assayed values. There were no significant changes in CFU-GM at any stage. Studies of T cell function are in progress to evaluate the post-thaw decline in assayed T cells. These data suggest that PBSC can be held at RT for up to 2 hours after thawing with no evident damage to progenitor and T cell populations beyond losses occurring after the initial freeze/thaw process.

US$29.90


Other references

Li, X-ping.; Qi, J-ying.; Wang, C-lin.; Chen, Y-heng., 2012: Distribution of heavy metals in waters and pollution assessment in thallium contaminated area of Yunfu, Guangdong. Distribution of Thallium(T1), Cadmium( Cd), Chromium (Cr), Lead (Pb), Nickel (Ni), Arsenic (As), Copper (Cu), Zinc (Zn) in water and sediments of Yunfu pyrite mine area was studied. The environmental risk assessment was conducted systematically us...

Unissa, A.Nusrath.; Subbian, S.; Hanna, L.Elizabeth.; Selvakumar, N., 2016: Overview on mechanisms of isoniazid action and resistance in Mycobacterium tuberculosis. NlmCategory="UNASSIGNED">Isoniazid (INH) is one of the most active compounds used to treat tuberculosis (TB) worldwide. In addition, INH has been used as a prophylactic drug for individuals with latent Mycobacterium tuberculosis (MTB)...

Jakubcová, I.; Michalko, J.; Cernay, J., 1977: Rheumatic diseases in children. Casopis Lekaru Ceskych 116(11): 326-331

Vyslozil, O., 1971: Problems of prosthetic provision in a case of eosinophilic granuloma. Oesterreichische Dentisten Zeitschrift 23(10): 233-238

Sun, P-Lun.; Peng, P-Chieh.; Wu, P-Hsien.; Chiang, Y-Lun.; Ju, Y-Ming.; Chang, C-Chi.; Wang, P-Cheng., 2013: Canine eumycetoma caused by Cladophialophora bantiana in a Maltese: case report and literature review. Mycoses 56(3): 376-381

Kitagawa, T.; Okita, T.; Banno, Y.; Sugiyama, S.; Okazaki, T.; Yoshioka, M.; Kashiwagi, M., 2000: Mitochondrial DNA of the Florida subspecies of largemouth bass Micropterus salmoides floridanus detected in Ikehara Reservoir, Nara Prefecture, Japan. The largemouth bass Micropterus salmoides consists of two subspecies M.s. salmoides and the Florida subspecies M.s. floridanus. It has been widely regarded that largemouth bass, introduced and widespread in Japan was the northern subspecies. In 19...

MacLeod, K.G.; Huber, B.T.; Bice, K.L.; Jimenez-Berrocoso, A., 2008: Turonian subtropical paleotemperatures from glassy Foraminifera in East Africa and their implications for modeling greenhouse climates. Specimens of "glassy" foraminifera from southern coastal Tanzania indicate sea surface temperatures up to 34 degrees C for subtropical shelfal waters (assuming a seawater delta18 O value of -1ppm(SMOW) ) and a consistent planktic tempera...

Zhou, H-Jun.; Tang, T.; Zhong, J-Hua.; Luo, J-Kun.; Cui, H-Jin.; Zhang, Q-Mei.; Zhou, J-Hua.; Zhang, Q., 2015: Electroacupuncture improves recovery after hemorrhagic brain injury by inducing the expression of angiopoietin-1 and -2 in rats. Angiopoietin (Ang) is one of the major effectors of angiogenesis, playing a critical role in neurovascular remodeling after stroke. Acupuncture has been widely used for treating stroke in China for a long time. Recently, we have demonstrated that...

Rangone, R.; L.F.ta, S., 1966: Applications of gas chromatography to the determination of organic solvents contained in products for pharmaceutical use. Il Farmaco; Edizione Pratica 21(1): 30-39

Marks, B.L.; Rippe, J.M., 1996: The importance of fat free mass maintenance in weight loss programmes. Obese individuals have excess total body mass, a condition resulting from an overaccumulation of both fat and fat free mass (FFM). Research has been focusing on the need to maintain FFM during weight loss because of its integral role in metabolic...