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Quantitative determination of allicin in Allium sativum L bulbs by UPLC

, : Quantitative determination of allicin in Allium sativum L bulbs by UPLC. Chromatographia: 1 2, 159-161

An UPLC method for determination of allicin in garlic bulb powder has been developed and validated. Chromatographic separation and determination were performed on an Acquity UPLC BEH C<sub>18</sub> column using a mobile phase of methanol/water (50:50, <i>v/v</i>). Linearity between the allicin concentration and the peak area was good within the concentration ranges 2.04-510 mg L<sup>-1</sup>. The method was validated over this range for both accuracy and precision.


DOI: 10.1365/s10337-009-1416-1

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